GroEL1: A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria

Anil Ojha, Mridula Anand, Apoorva Bhatt, Laurent Kremer, William R. Jacobs, Graham F. Hatfull

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential - presumably providing the housekeeping chaperone functions - while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of the Mycobacterium smegmatis groEL1 gene by phage Bxb1 integration allows normal planktonic growth but prevents the formation of mature biofilms. GroEL1 modulates synthesis of mycolates - long-chain fatty acid components of the mycobacterial cell wall - specifically during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Biofilm formation is associated with elevated synthesis of short-chain (C56-C 68) fatty acids, and strains with altered mycolate profiles - including an InhA mutant resistant to the antituberculosis drug isoniazid and a strain overexpressing KasA - are defective in biofilm formation.

Original languageEnglish (US)
Pages (from-to)861-873
Number of pages13
JournalCell
Volume123
Issue number5
DOIs
StatePublished - Dec 2 2005

Fingerprint

Mycolic Acids
Biosynthesis
Biofilms
Mycobacterium
Bacteriophages
Type II Fatty Acid Synthase
Fatty Acids
Mycobacterium smegmatis
Housekeeping
Isoniazid
Cell Wall
Genes
Cells
Growth
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

GroEL1 : A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria. / Ojha, Anil; Anand, Mridula; Bhatt, Apoorva; Kremer, Laurent; Jacobs, William R.; Hatfull, Graham F.

In: Cell, Vol. 123, No. 5, 02.12.2005, p. 861-873.

Research output: Contribution to journalArticle

Ojha, Anil ; Anand, Mridula ; Bhatt, Apoorva ; Kremer, Laurent ; Jacobs, William R. ; Hatfull, Graham F. / GroEL1 : A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria. In: Cell. 2005 ; Vol. 123, No. 5. pp. 861-873.
@article{9834beba9f0d4ba194eab08e9e1e55e0,
title = "GroEL1: A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria",
abstract = "Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential - presumably providing the housekeeping chaperone functions - while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of the Mycobacterium smegmatis groEL1 gene by phage Bxb1 integration allows normal planktonic growth but prevents the formation of mature biofilms. GroEL1 modulates synthesis of mycolates - long-chain fatty acid components of the mycobacterial cell wall - specifically during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Biofilm formation is associated with elevated synthesis of short-chain (C56-C 68) fatty acids, and strains with altered mycolate profiles - including an InhA mutant resistant to the antituberculosis drug isoniazid and a strain overexpressing KasA - are defective in biofilm formation.",
author = "Anil Ojha and Mridula Anand and Apoorva Bhatt and Laurent Kremer and Jacobs, {William R.} and Hatfull, {Graham F.}",
year = "2005",
month = "12",
day = "2",
doi = "10.1016/j.cell.2005.09.012",
language = "English (US)",
volume = "123",
pages = "861--873",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - GroEL1

T2 - A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria

AU - Ojha, Anil

AU - Anand, Mridula

AU - Bhatt, Apoorva

AU - Kremer, Laurent

AU - Jacobs, William R.

AU - Hatfull, Graham F.

PY - 2005/12/2

Y1 - 2005/12/2

N2 - Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential - presumably providing the housekeeping chaperone functions - while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of the Mycobacterium smegmatis groEL1 gene by phage Bxb1 integration allows normal planktonic growth but prevents the formation of mature biofilms. GroEL1 modulates synthesis of mycolates - long-chain fatty acid components of the mycobacterial cell wall - specifically during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Biofilm formation is associated with elevated synthesis of short-chain (C56-C 68) fatty acids, and strains with altered mycolate profiles - including an InhA mutant resistant to the antituberculosis drug isoniazid and a strain overexpressing KasA - are defective in biofilm formation.

AB - Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential - presumably providing the housekeeping chaperone functions - while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of the Mycobacterium smegmatis groEL1 gene by phage Bxb1 integration allows normal planktonic growth but prevents the formation of mature biofilms. GroEL1 modulates synthesis of mycolates - long-chain fatty acid components of the mycobacterial cell wall - specifically during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Biofilm formation is associated with elevated synthesis of short-chain (C56-C 68) fatty acids, and strains with altered mycolate profiles - including an InhA mutant resistant to the antituberculosis drug isoniazid and a strain overexpressing KasA - are defective in biofilm formation.

UR - http://www.scopus.com/inward/record.url?scp=28344453690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28344453690&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2005.09.012

DO - 10.1016/j.cell.2005.09.012

M3 - Article

C2 - 16325580

AN - SCOPUS:28344453690

VL - 123

SP - 861

EP - 873

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -