Abstract
Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1648-1657 |
| Number of pages | 10 |
| Journal | Free Radical Biology and Medicine |
| Volume | 52 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 1 2012 |
| Externally published | Yes |
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Keywords
- Adipocyte
- Diabetes
- EGCG
- Green tea catechins
- Insulin resistance
- Reactive oxidative species (ROS)
ASJC Scopus subject areas
- Biochemistry
- Physiology (medical)
Cite this
Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress. / Yan, Jingqi; Zhao, Yan; Suo, Siqingaowa; Liu, Yang; Zhao, Baolu.
In: Free Radical Biology and Medicine, Vol. 52, No. 9, 01.05.2012, p. 1648-1657.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress
AU - Yan, Jingqi
AU - Zhao, Yan
AU - Suo, Siqingaowa
AU - Liu, Yang
AU - Zhao, Baolu
PY - 2012/5/1
Y1 - 2012/5/1
N2 - Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.
AB - Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.
KW - Adipocyte
KW - Diabetes
KW - EGCG
KW - Green tea catechins
KW - Insulin resistance
KW - Reactive oxidative species (ROS)
UR - http://www.scopus.com/inward/record.url?scp=84858992662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84858992662&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2012.01.033
DO - 10.1016/j.freeradbiomed.2012.01.033
M3 - Article
C2 - 22330066
AN - SCOPUS:84858992662
VL - 52
SP - 1648
EP - 1657
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 9
ER -