Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress

Jingqi Yan, Yan Zhao, Siqingaowa Suo, Yang Liu, Baolu Zhao

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.

Original languageEnglish (US)
Pages (from-to)1648-1657
Number of pages10
JournalFree Radical Biology and Medicine
Volume52
Issue number9
DOIs
StatePublished - May 1 2012
Externally publishedYes

Fingerprint

Oxidative stress
Catechin
Tea
Insulin Resistance
Oxidative Stress
Insulin
Adipocytes
Glucose
Animals
Assays
High Fat Diet
Phosphorylation
Scavenging
Dexamethasone
Drinking
Nutrition
Medical problems
Animal Models
Rats
Fats

Keywords

  • Adipocyte
  • Diabetes
  • EGCG
  • Green tea catechins
  • Insulin resistance
  • Reactive oxidative species (ROS)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress. / Yan, Jingqi; Zhao, Yan; Suo, Siqingaowa; Liu, Yang; Zhao, Baolu.

In: Free Radical Biology and Medicine, Vol. 52, No. 9, 01.05.2012, p. 1648-1657.

Research output: Contribution to journalArticle

Yan, Jingqi ; Zhao, Yan ; Suo, Siqingaowa ; Liu, Yang ; Zhao, Baolu. / Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress. In: Free Radical Biology and Medicine. 2012 ; Vol. 52, No. 9. pp. 1648-1657.
@article{c14f3590d9cb4a1ea62e5c502df903a8,
title = "Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress",
abstract = "Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.",
keywords = "Adipocyte, Diabetes, EGCG, Green tea catechins, Insulin resistance, Reactive oxidative species (ROS)",
author = "Jingqi Yan and Yan Zhao and Siqingaowa Suo and Yang Liu and Baolu Zhao",
year = "2012",
month = "5",
day = "1",
doi = "10.1016/j.freeradbiomed.2012.01.033",
language = "English (US)",
volume = "52",
pages = "1648--1657",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Green tea catechins ameliorate adipose insulin resistance by improving oxidative stress

AU - Yan, Jingqi

AU - Zhao, Yan

AU - Suo, Siqingaowa

AU - Liu, Yang

AU - Zhao, Baolu

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.

AB - Epidemiological data have suggested that drinking green tea is negatively associated with diabetes, and adipose oxidative stress may have a central role in causing insulin resistance, according to recent findings. The aim of this work is to elucidate a new mechanism for green tea's anti-insulin resistance effect. We used obese KK-ay mice, high-fat diet-induced obese rats, and induced insulin resistant 3T3-L1 adipocytes as models. Insulin sensitivity and adipose reactive oxidative species (ROS) levels were detected in animals and adipocytes. The oxidative stress assay and glucose uptake ability assay were performed, and the effects of EGCG on insulin signals were detected. Green tea catechins (GTCs) significantly decreased glucose levels and increased glucose tolerance in animals. GTCs reduced ROS content in both models of animal and adipocytes. EGCG attenuated dexamethasone and TNF-α promoted ROS generation and increased glucose uptake ability. EGCG also decreased JNK phosphorylation and promoted GLUT-4 translocation. EGCG and GTCs could improve adipose insulin resistance, and exact this effect on their ROS scavenging functions.

KW - Adipocyte

KW - Diabetes

KW - EGCG

KW - Green tea catechins

KW - Insulin resistance

KW - Reactive oxidative species (ROS)

UR - http://www.scopus.com/inward/record.url?scp=84858992662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858992662&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2012.01.033

DO - 10.1016/j.freeradbiomed.2012.01.033

M3 - Article

C2 - 22330066

AN - SCOPUS:84858992662

VL - 52

SP - 1648

EP - 1657

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 9

ER -