Granuloma formation around filarial larvae triggered by host responses to an excretory/secretory antigen

Yashodhara Dash, Manish Ramesh, Ramaswamy Kalyanasundaram, Gnanasekar Munirathinam, Leonard D. Shultz, T. V. Rajan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In previous studies using a murine model of filarial infection, granuloma formation was found to be a most important host-protective mechanism. We have also shown that in vitro cytoadherence is a surrogate for the formation of antifilarial granulomas in vivo and that it requires "alternatively activated" host cells and a source of antifilarial antibody. We show here that antibodies against L3 excretory/secretory (E/S) products can facilitate in vitro cytoadherence. We generated a set of hybridomas reactive with filarial E/S products and screened them for their ability to mediate in vitro cytoadherence. One clone (no. 1E9) was positive in this assay. We then screened a novel expression library of filarial antigens displayed on the surface of T7 bacteriophage for reactivity with 1E9. Phage expressing two filarial antigens (TCTP and BmALT-2) reacted with 1E9. Immunization of mice showed that the cohort immunized with BmALT-2 cleared a challenge infection with infective Brugia pahangi L3 in an accelerated manner, whereas cohorts immunized with TCTP cleared larvae with the same kinetics as in unimmunized mice. These data confirm that BmALT-2 is the antigenic target of granuloma-mediated killing of B. pahangi L3. Our findings also confirm previous studies that BmALT-2 is a potential vaccine candidate for filarial infection. Our data reinforce the work of others and also provide a possible mechanism by which immune responses to BmALT-2 may provide host protection.

Original languageEnglish (US)
Pages (from-to)838-845
Number of pages8
JournalInfection and immunity
Volume79
Issue number2
DOIs
StatePublished - Feb 2011

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Granuloma formation around filarial larvae triggered by host responses to an excretory/secretory antigen'. Together they form a unique fingerprint.

  • Cite this