Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release

Todd D. Schraw, Tara W. Rutledge, Garland L. Crawford, Audrey M. Bernstein, Amanda L. Kalen, Jeffrey E. Pessin, Sidney W. Whiteheart

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

It is widely accepted that the platelet release reaction is mediated by heterotrimeric complexes of integral membrane proteins known as SNAREs (SNAP receptors). In an effort to define the precise molecular machinery required for platelet exocytosis, we have analyzed platelets from cellubrevin/VAMP-3 knockout mice. Cellubrevin/VAMP-3 has been proposed to be a critical v-SNARE for human platelet exocytosis; however, data reported here suggest that it is not required for platelet function. Upon stimulation with increasing concentrations of thrombin, collagen, or with thrombin for increasing time there were no differences in secretion of [3H]-5HT (dense core granules), platelet factor IV (alpha granules), or hexosaminidase (lysosomes) between null and wild-type platelets. There were no gross differences in bleeding times nor in agonist-induced aggregation measured in platelet-rich plasma or with washed platelets. Western blotting of wild-type, heterozygous, and null platelets confirmed the lack of cellubrevin/VAMP-3 in nulls and showed that most elements of the secretion machinery are expressed at similar levels. While the secretory machinery in mice was similar to humans, mice did express apparently higher levels of synaptobrevin/VAMP-2. These data show that the v-SNARE, cellubrevin/VAMP-3 is not a requirement for the platelet release reaction in mice.

Original languageEnglish (US)
Pages (from-to)1716-1722
Number of pages7
JournalBlood
Volume102
Issue number5
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

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Vesicle-Associated Membrane Protein 3
Platelets
Blood Platelets
SNARE Proteins
Vesicle-Associated Membrane Protein 2
Machinery
Exocytosis
Thrombin
R-SNARE Proteins
Thrombin Time
Hexosaminidases
Bleeding Time
Platelet-Rich Plasma
Lysosomes
Knockout Mice

ASJC Scopus subject areas

  • Hematology

Cite this

Schraw, T. D., Rutledge, T. W., Crawford, G. L., Bernstein, A. M., Kalen, A. L., Pessin, J. E., & Whiteheart, S. W. (2003). Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. Blood, 102(5), 1716-1722. https://doi.org/10.1182/blood-2003-01-0331

Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. / Schraw, Todd D.; Rutledge, Tara W.; Crawford, Garland L.; Bernstein, Audrey M.; Kalen, Amanda L.; Pessin, Jeffrey E.; Whiteheart, Sidney W.

In: Blood, Vol. 102, No. 5, 01.09.2003, p. 1716-1722.

Research output: Contribution to journalArticle

Schraw, TD, Rutledge, TW, Crawford, GL, Bernstein, AM, Kalen, AL, Pessin, JE & Whiteheart, SW 2003, 'Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release', Blood, vol. 102, no. 5, pp. 1716-1722. https://doi.org/10.1182/blood-2003-01-0331
Schraw, Todd D. ; Rutledge, Tara W. ; Crawford, Garland L. ; Bernstein, Audrey M. ; Kalen, Amanda L. ; Pessin, Jeffrey E. ; Whiteheart, Sidney W. / Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. In: Blood. 2003 ; Vol. 102, No. 5. pp. 1716-1722.
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