Gpr125 modulates Dishevelled distribution and planar cell polarity signaling

Xin Li, Isabelle Roszko, Diane S. Sepich, Mingwei Ni, Heidi E. Hamm, Florence L. Marlow, Lilianna Solnica-Krezel

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

During vertebrate gastrulation, Wnt/planar cell polarity (PCP) signaling orchestrates polarized cell behaviors underlying convergence and extension (C&E) movements to narrow embryonic tissues mediolaterally and lengthen them anteroposteriorly. Here, we have identified Gpr125, an adhesion G protein-coupled receptor, as a novel modulator of the Wnt/PCP signaling system. Excess Gpr125 impaired C&E movements and the underlying cell and molecular polarities. Reduced Gpr125 function exacerbated the C&E and facial branchiomotor neuron (FBMN) migration defects of embryos with reduced Wnt/PCP signaling. At the molecular level, Gpr125 recruited Dishevelled to the cell membrane, a prerequisite for Wnt/PCP activation. Moreover, Gpr125 and Dvl mutually clustered one another to form discrete membrane subdomains, and the Gpr125 intracellular domain directly interacted with Dvl in pull-down assays. Intriguingly, Dvl and Gpr125 were able to recruit a subset of PCP components into membrane subdomains, suggesting that Gpr125 may modulate the composition of Wnt/PCP membrane complexes. Our study reveals a role for Gpr125 in PCP-mediated processes and provides mechanistic insight into Wnt/PCP signaling.

Original languageEnglish (US)
Pages (from-to)3028-3039
Number of pages12
JournalDevelopment (Cambridge)
Volume140
Issue number14
DOIs
StatePublished - Jul 15 2013

Keywords

  • Convergence and extension
  • Facial branchiomotor neuron
  • Gastrulation movements
  • Zebrafish

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Li, X., Roszko, I., Sepich, D. S., Ni, M., Hamm, H. E., Marlow, F. L., & Solnica-Krezel, L. (2013). Gpr125 modulates Dishevelled distribution and planar cell polarity signaling. Development (Cambridge), 140(14), 3028-3039. https://doi.org/10.1242/dev.094839