GPCR signaling mediates tumor metastasis via PI3Kβ

Bassem D. Khalil, Christine Hsueh, Yanyan Cao, Widian F.Abi Saab, Yarong Wang, John S. Condeelis, Anne R. Bresnick, Jonathan M. Backer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Inappropriate activation of PI3K signaling has been implicated strongly in human cancer. Although studies on the role of PI3K signaling in breast tumorigenesis and progression have focused most intensively on PI3Ka, a role for PI3Kα has begun to emerge. The PI3Kβ isoform is unique among class IA PI3K enzymes in that it is activated by both receptor tyrosine kinases and G-protein- coupled receptors (GPCR). In previous work, we identified a mutation that specifically abolishes PI3Kβ binding to Gβγ (p110526KK-DD). Expression of this mutant in p110β-silenced breast cancer cells inhibits multiple steps of the metastatic cascade in vitro and in vivo and causes a cell autonomous defect in invadopodial matrix degradation. Our results identify a novel link between GPCRs and PI3Kβ in mediating metastasis, suggesting that disruption of this link might offer a novel therapeutic target to prevent the development of metastatic disease.

Original languageEnglish (US)
Pages (from-to)2944-2953
Number of pages10
JournalCancer Research
Volume76
Issue number10
DOIs
StatePublished - May 15 2016

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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