Gossypol, a hyperthermic sensitizer of HeLa cells

S. H. Kim, J. H. Kim, A. A. Alfieri, S. Q. He, C. W. Young

Research output: Contribution to journalArticle

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Abstract

Gossypol, a polyphenolic aldehyde extracted from cotton plants, is a potent antifertility agent in humans. Since we have previously reported that several male antifertility agents including 5-thio-D-glucose and lonidamine demonstrate hyperthermic sensitizing effects in HeLa cells, we wished to determine whether gossypol also exhibits the hyperthermic sensitization. Gossypol was not cytotoxic up to 4 h at 37°C (10 μg/ml). When HeLa cells were exposed to gossypol at 41° and 42° C, significant potentiation of hyperthermia induced cytotoxicity was observed. The magnitude of the potentiation was dependent on the drug concentration, pH of the cultures medium, glucose concentration, temperature, and duration of treatment. The hyperthermic sensitizing effect of gossypol was increased by an acidic pH and glucose deprivation. These data suggest that the sensitizing effect of the drug may be mediated through the lowering of cellular energy level by the inhibition of both glycolysis and oxidative phosphorylation.

Original languageEnglish (US)
Pages (from-to)6338-6340
Number of pages3
JournalCancer Research
Volume45
Issue number12
StatePublished - 1985
Externally publishedYes

Fingerprint

Gossypol
HeLa Cells
Gossypium
Glucose
Induced Hyperthermia
Oxidative Phosphorylation
Glycolysis
Aldehydes
Pharmaceutical Preparations
Culture Media
Temperature

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kim, S. H., Kim, J. H., Alfieri, A. A., He, S. Q., & Young, C. W. (1985). Gossypol, a hyperthermic sensitizer of HeLa cells. Cancer Research, 45(12), 6338-6340.

Gossypol, a hyperthermic sensitizer of HeLa cells. / Kim, S. H.; Kim, J. H.; Alfieri, A. A.; He, S. Q.; Young, C. W.

In: Cancer Research, Vol. 45, No. 12, 1985, p. 6338-6340.

Research output: Contribution to journalArticle

Kim, SH, Kim, JH, Alfieri, AA, He, SQ & Young, CW 1985, 'Gossypol, a hyperthermic sensitizer of HeLa cells', Cancer Research, vol. 45, no. 12, pp. 6338-6340.
Kim SH, Kim JH, Alfieri AA, He SQ, Young CW. Gossypol, a hyperthermic sensitizer of HeLa cells. Cancer Research. 1985;45(12):6338-6340.
Kim, S. H. ; Kim, J. H. ; Alfieri, A. A. ; He, S. Q. ; Young, C. W. / Gossypol, a hyperthermic sensitizer of HeLa cells. In: Cancer Research. 1985 ; Vol. 45, No. 12. pp. 6338-6340.
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