Going viral with fluorescent proteins

Lindsey M. Costantini, Erik L. Snapp

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges of and offer suggestions for identifying the optimal FPs for studying the cell biology of viruses.

Original languageEnglish (US)
Pages (from-to)9706-9708
Number of pages3
JournalJournal of Virology
Volume89
Issue number19
DOIs
StatePublished - 2015

Fingerprint

Proteins
Viruses
viruses
Optical Imaging
viral proteins
Viral Proteins
Cell Communication
cell biology
Cell Biology
physical properties
physicochemical properties
image analysis
fluorescence
fluorescent proteins
cells
methodology
protein tagging

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Costantini, L. M., & Snapp, E. L. (2015). Going viral with fluorescent proteins. Journal of Virology, 89(19), 9706-9708. https://doi.org/10.1128/JVI.03489-13

Going viral with fluorescent proteins. / Costantini, Lindsey M.; Snapp, Erik L.

In: Journal of Virology, Vol. 89, No. 19, 2015, p. 9706-9708.

Research output: Contribution to journalArticle

Costantini, LM & Snapp, EL 2015, 'Going viral with fluorescent proteins', Journal of Virology, vol. 89, no. 19, pp. 9706-9708. https://doi.org/10.1128/JVI.03489-13
Costantini, Lindsey M. ; Snapp, Erik L. / Going viral with fluorescent proteins. In: Journal of Virology. 2015 ; Vol. 89, No. 19. pp. 9706-9708.
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