Abstract
Many longstanding questions about dynamics of virus-cell interactions can be answered by combining fluorescence imaging techniques with fluorescent protein (FP) tagging strategies. Successfully creating a FP fusion with a cellular or viral protein of interest first requires selecting the appropriate FP. However, while viral architecture and cellular localization often dictate the suitability of a FP, a FP's chemical and physical properties must also be considered. Here, we discuss the challenges of and offer suggestions for identifying the optimal FPs for studying the cell biology of viruses.
Original language | English (US) |
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Pages (from-to) | 9706-9708 |
Number of pages | 3 |
Journal | Journal of virology |
Volume | 89 |
Issue number | 19 |
DOIs | |
State | Published - 2015 |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology