Glycosylation of the major polar tube protein of Encephalitozoon hellem, a microsporidian parasite that infects humans

Yanji Xu, Peter M. Takvorian, Ann Cali, George Orr, Louis M. Weiss

Research output: Contribution to journalArticle

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Abstract

The microsporidia are ubiquitous, obligate intracellular eukaryotic spore-forming parasites infecting a wide range of invertebrates and vertebrates, including humans. The defining structure of microsporidia is the polar tube, which forms a hollow tube through which the sporoplasm is transferred to the host cell. Research on the molecular and cellular biology of the polar tube has resulted in the identification of three polar tube proteins: PTP1, PTP2, and PTP3. The major polar tube protein, PTP1, accounts for at least 70% of the mass of the polar tube. In the present study, PTP1 was found to be posttranslationally modified. Concanavalin A (ConA) bound to PTP1 and to the polar tube of several different microsporidia species. Analysis of the glycosylation of Encephalitozoon hellem PTP1 suggested that it is modified by O-linked mannosylation, and ConA binds to these O-linked mannose residues. Mannose pretreatment of RK13 host cells decreased their infection by E. hellem, consistent with an interaction between the mannosylation of PTP1 and some unknown host cell mannose-binding molecule. A CHO cell line (Lec1) that is unable to synthesize complex-type N-linked oligosaccharides had an increased susceptibility to E. hellem infection compared to wild-type CHO cells. These data suggest that the O-mannosylation of PTP1 may have functional significance for the ability of microsporidia to invade their host cells.

Original languageEnglish (US)
Pages (from-to)6341-6350
Number of pages10
JournalInfection and Immunity
Volume72
Issue number11
DOIs
StatePublished - Nov 2004

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Microsporidia
Glycosylation
Parasites
Mannose
CHO Cells
Concanavalin A
Encephalitozoon
Invertebrates
Infection
Spores
Oligosaccharides
Cell Biology
Vertebrates
Molecular Biology
Proteins
Cell Line
Encephalitozoon hellem PTP protein
Research

ASJC Scopus subject areas

  • Immunology

Cite this

Glycosylation of the major polar tube protein of Encephalitozoon hellem, a microsporidian parasite that infects humans. / Xu, Yanji; Takvorian, Peter M.; Cali, Ann; Orr, George; Weiss, Louis M.

In: Infection and Immunity, Vol. 72, No. 11, 11.2004, p. 6341-6350.

Research output: Contribution to journalArticle

Xu, Yanji ; Takvorian, Peter M. ; Cali, Ann ; Orr, George ; Weiss, Louis M. / Glycosylation of the major polar tube protein of Encephalitozoon hellem, a microsporidian parasite that infects humans. In: Infection and Immunity. 2004 ; Vol. 72, No. 11. pp. 6341-6350.
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