Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues

Immunochemical characterization, subcellular localization, and regulation of biosynthesis

A. Laslop, H. J. Steiner, C. Egger, M. Wolkersdorfer, S. Kapelari, R. Hogue-Angeletti, J. D. Erickson, R. Fischer-Colbrie, H. Winkler

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.

Original languageEnglish (US)
Pages (from-to)1498-1505
Number of pages8
JournalJournal of Neurochemistry
Volume61
Issue number4
StatePublished - Oct 1993

Fingerprint

Clusterin
Nerve Tissue
Biosynthesis
Glycoproteins
Tissue
Secretogranin II
Chromaffin Cells
Adrenal Medulla
Immunoelectron Microscopy
Secretory Vesicles
Tetradecanoylphorbol Acetate
Colforsin
Fractionation
Electrophoresis
Immunoblotting
Organelles
Liver
Histamine
Immune Sera
Brain

Keywords

  • Adrenal medulla
  • Clusterin
  • Large dense-core vesicles
  • Pituitary gland

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues : Immunochemical characterization, subcellular localization, and regulation of biosynthesis. / Laslop, A.; Steiner, H. J.; Egger, C.; Wolkersdorfer, M.; Kapelari, S.; Hogue-Angeletti, R.; Erickson, J. D.; Fischer-Colbrie, R.; Winkler, H.

In: Journal of Neurochemistry, Vol. 61, No. 4, 10.1993, p. 1498-1505.

Research output: Contribution to journalArticle

Laslop, A, Steiner, HJ, Egger, C, Wolkersdorfer, M, Kapelari, S, Hogue-Angeletti, R, Erickson, JD, Fischer-Colbrie, R & Winkler, H 1993, 'Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues: Immunochemical characterization, subcellular localization, and regulation of biosynthesis', Journal of Neurochemistry, vol. 61, no. 4, pp. 1498-1505.
Laslop, A. ; Steiner, H. J. ; Egger, C. ; Wolkersdorfer, M. ; Kapelari, S. ; Hogue-Angeletti, R. ; Erickson, J. D. ; Fischer-Colbrie, R. ; Winkler, H. / Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues : Immunochemical characterization, subcellular localization, and regulation of biosynthesis. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 4. pp. 1498-1505.
@article{e2a4de5531a34e0ab1bc97b0951adc98,
title = "Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues: Immunochemical characterization, subcellular localization, and regulation of biosynthesis",
abstract = "Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.",
keywords = "Adrenal medulla, Clusterin, Large dense-core vesicles, Pituitary gland",
author = "A. Laslop and Steiner, {H. J.} and C. Egger and M. Wolkersdorfer and S. Kapelari and R. Hogue-Angeletti and Erickson, {J. D.} and R. Fischer-Colbrie and H. Winkler",
year = "1993",
month = "10",
language = "English (US)",
volume = "61",
pages = "1498--1505",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Glycoprotein III (clusterin, sulfated glycoprotein 2) in endocrine, nervous, and other tissues

T2 - Immunochemical characterization, subcellular localization, and regulation of biosynthesis

AU - Laslop, A.

AU - Steiner, H. J.

AU - Egger, C.

AU - Wolkersdorfer, M.

AU - Kapelari, S.

AU - Hogue-Angeletti, R.

AU - Erickson, J. D.

AU - Fischer-Colbrie, R.

AU - Winkler, H.

PY - 1993/10

Y1 - 1993/10

N2 - Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.

AB - Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.

KW - Adrenal medulla

KW - Clusterin

KW - Large dense-core vesicles

KW - Pituitary gland

UR - http://www.scopus.com/inward/record.url?scp=0027490902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027490902&partnerID=8YFLogxK

M3 - Article

VL - 61

SP - 1498

EP - 1505

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -