Glycogen depletion can increase the specificity of mucin detection in airway tissues

David K. Meyerholz, Amanda P. Beck, J. Adam Goeken, Mariah R. Leidinger, Georgina K. Ofori-Amanfo, Hannah C. Brown, Thomas R. Businga, David A. Stoltz, Leah R. Reznikov, Heather A. Flaherty

Research output: Contribution to journalArticle

Abstract

Objective: Mucin is an important parameter for detection and assessment in studies of airway disease including asthma and cystic fibrosis. Histochemical techniques are often used to evaluate mucin in tissues sections. Periodic acid Schiff (PAS) is a common technique to detect neutral mucins in tissue, but this technique also detects other tissue components including cellular glycogen. We tested whether depletion of glycogen, a common cellular constituent, could impact the detection of mucin in the surface epithelium of the trachea. Results: Normal tissues stained by PAS had significantly more staining than serial sections of glycogen-depleted tissue with PAS staining (i.e. dPAS technique) based on both quantitative analysis and semiquantitative scores. Most of the excess stain by the PAS technique was detected in ciliated cells adjacent to goblet cells. We also compared normal tissues using the Alcian blue technique, which does not have reported glycogen staining, with the dPAS technique. These groups had similar amounts of staining consistent with a high degree of mucin specificity. Our results suggest that when using PAS techniques to stain airways, the dPAS approach is preferred as it enhances the specificity for airway mucin.

Original languageEnglish (US)
Article number763
JournalBMC Research Notes
Volume11
Issue number1
DOIs
StatePublished - Oct 25 2018

Fingerprint

Mucins
Periodic Acid
Glycogen
Tissue
Staining and Labeling
Coloring Agents
Alcian Blue
Goblet Cells
Trachea
Cystic Fibrosis
Asthma
Epithelium
Chemical analysis

Keywords

  • Alcian blue
  • Diastase-periodic acid Schiff (dPAS)
  • Glycogen
  • Mucin
  • Mucus
  • Nonspecific staining
  • Periodic acid Schiff (PAS)
  • Scoring
  • Specificity
  • Staining

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Meyerholz, D. K., Beck, A. P., Goeken, J. A., Leidinger, M. R., Ofori-Amanfo, G. K., Brown, H. C., ... Flaherty, H. A. (2018). Glycogen depletion can increase the specificity of mucin detection in airway tissues. BMC Research Notes, 11(1), [763]. https://doi.org/10.1186/s13104-018-3855-y

Glycogen depletion can increase the specificity of mucin detection in airway tissues. / Meyerholz, David K.; Beck, Amanda P.; Goeken, J. Adam; Leidinger, Mariah R.; Ofori-Amanfo, Georgina K.; Brown, Hannah C.; Businga, Thomas R.; Stoltz, David A.; Reznikov, Leah R.; Flaherty, Heather A.

In: BMC Research Notes, Vol. 11, No. 1, 763, 25.10.2018.

Research output: Contribution to journalArticle

Meyerholz, DK, Beck, AP, Goeken, JA, Leidinger, MR, Ofori-Amanfo, GK, Brown, HC, Businga, TR, Stoltz, DA, Reznikov, LR & Flaherty, HA 2018, 'Glycogen depletion can increase the specificity of mucin detection in airway tissues', BMC Research Notes, vol. 11, no. 1, 763. https://doi.org/10.1186/s13104-018-3855-y
Meyerholz, David K. ; Beck, Amanda P. ; Goeken, J. Adam ; Leidinger, Mariah R. ; Ofori-Amanfo, Georgina K. ; Brown, Hannah C. ; Businga, Thomas R. ; Stoltz, David A. ; Reznikov, Leah R. ; Flaherty, Heather A. / Glycogen depletion can increase the specificity of mucin detection in airway tissues. In: BMC Research Notes. 2018 ; Vol. 11, No. 1.
@article{d8cec53c2c7e45ecb6762191ff7851f6,
title = "Glycogen depletion can increase the specificity of mucin detection in airway tissues",
abstract = "Objective: Mucin is an important parameter for detection and assessment in studies of airway disease including asthma and cystic fibrosis. Histochemical techniques are often used to evaluate mucin in tissues sections. Periodic acid Schiff (PAS) is a common technique to detect neutral mucins in tissue, but this technique also detects other tissue components including cellular glycogen. We tested whether depletion of glycogen, a common cellular constituent, could impact the detection of mucin in the surface epithelium of the trachea. Results: Normal tissues stained by PAS had significantly more staining than serial sections of glycogen-depleted tissue with PAS staining (i.e. dPAS technique) based on both quantitative analysis and semiquantitative scores. Most of the excess stain by the PAS technique was detected in ciliated cells adjacent to goblet cells. We also compared normal tissues using the Alcian blue technique, which does not have reported glycogen staining, with the dPAS technique. These groups had similar amounts of staining consistent with a high degree of mucin specificity. Our results suggest that when using PAS techniques to stain airways, the dPAS approach is preferred as it enhances the specificity for airway mucin.",
keywords = "Alcian blue, Diastase-periodic acid Schiff (dPAS), Glycogen, Mucin, Mucus, Nonspecific staining, Periodic acid Schiff (PAS), Scoring, Specificity, Staining",
author = "Meyerholz, {David K.} and Beck, {Amanda P.} and Goeken, {J. Adam} and Leidinger, {Mariah R.} and Ofori-Amanfo, {Georgina K.} and Brown, {Hannah C.} and Businga, {Thomas R.} and Stoltz, {David A.} and Reznikov, {Leah R.} and Flaherty, {Heather A.}",
year = "2018",
month = "10",
day = "25",
doi = "10.1186/s13104-018-3855-y",
language = "English (US)",
volume = "11",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Glycogen depletion can increase the specificity of mucin detection in airway tissues

AU - Meyerholz, David K.

AU - Beck, Amanda P.

AU - Goeken, J. Adam

AU - Leidinger, Mariah R.

AU - Ofori-Amanfo, Georgina K.

AU - Brown, Hannah C.

AU - Businga, Thomas R.

AU - Stoltz, David A.

AU - Reznikov, Leah R.

AU - Flaherty, Heather A.

PY - 2018/10/25

Y1 - 2018/10/25

N2 - Objective: Mucin is an important parameter for detection and assessment in studies of airway disease including asthma and cystic fibrosis. Histochemical techniques are often used to evaluate mucin in tissues sections. Periodic acid Schiff (PAS) is a common technique to detect neutral mucins in tissue, but this technique also detects other tissue components including cellular glycogen. We tested whether depletion of glycogen, a common cellular constituent, could impact the detection of mucin in the surface epithelium of the trachea. Results: Normal tissues stained by PAS had significantly more staining than serial sections of glycogen-depleted tissue with PAS staining (i.e. dPAS technique) based on both quantitative analysis and semiquantitative scores. Most of the excess stain by the PAS technique was detected in ciliated cells adjacent to goblet cells. We also compared normal tissues using the Alcian blue technique, which does not have reported glycogen staining, with the dPAS technique. These groups had similar amounts of staining consistent with a high degree of mucin specificity. Our results suggest that when using PAS techniques to stain airways, the dPAS approach is preferred as it enhances the specificity for airway mucin.

AB - Objective: Mucin is an important parameter for detection and assessment in studies of airway disease including asthma and cystic fibrosis. Histochemical techniques are often used to evaluate mucin in tissues sections. Periodic acid Schiff (PAS) is a common technique to detect neutral mucins in tissue, but this technique also detects other tissue components including cellular glycogen. We tested whether depletion of glycogen, a common cellular constituent, could impact the detection of mucin in the surface epithelium of the trachea. Results: Normal tissues stained by PAS had significantly more staining than serial sections of glycogen-depleted tissue with PAS staining (i.e. dPAS technique) based on both quantitative analysis and semiquantitative scores. Most of the excess stain by the PAS technique was detected in ciliated cells adjacent to goblet cells. We also compared normal tissues using the Alcian blue technique, which does not have reported glycogen staining, with the dPAS technique. These groups had similar amounts of staining consistent with a high degree of mucin specificity. Our results suggest that when using PAS techniques to stain airways, the dPAS approach is preferred as it enhances the specificity for airway mucin.

KW - Alcian blue

KW - Diastase-periodic acid Schiff (dPAS)

KW - Glycogen

KW - Mucin

KW - Mucus

KW - Nonspecific staining

KW - Periodic acid Schiff (PAS)

KW - Scoring

KW - Specificity

KW - Staining

UR - http://www.scopus.com/inward/record.url?scp=85055613610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055613610&partnerID=8YFLogxK

U2 - 10.1186/s13104-018-3855-y

DO - 10.1186/s13104-018-3855-y

M3 - Article

VL - 11

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

IS - 1

M1 - 763

ER -