Abstract
Glutathione S-transferases (GSTs) are detoxification enzymes that can counter ageing-associated oxidative and chemical stresses. The transcript of a distinct subclass of human GSTs (hGSTM3) was shown by RNA blot analysis to be widely distributed in different regions of adult brain. HPLC profiles indicated that the hGSTM3 subunit was the second most abundant GST subunit in brain. Immunocytochemistry performed with hGSTM3-specific antisera, showed prominent staining of neuritic plaques, neurofibrillary tangles and microglia in sections of hippocampus obtained from patients with Alzheimer's disease. The staining pattern was distinct from that obtained with normal brains. Because hGSTM3 is rich in cysteine residues and readily undergoes S-glutathiolation reactions, deposition of this protein could originate from cross-links produced by oxidative stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 309-315 |
| Number of pages | 7 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 126 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2005 |
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Keywords
- Ageing
- Alzheimer's disease
- Brain
- Glutathione S-transferases
- hGSTM5 subunit
- Microglia
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
Glutathione S-transferase hGSTM3 and ageing-associated neurodegeneration : Relationship to Alzheimer's disease. / Tchaikovskaya, Tatyana L.; Fraifeld, Vadim; Urphanishvili, Tinatin; Andorfer, John H.; Davies, Peter; Listowsky, Irving.
In: Mechanisms of Ageing and Development, Vol. 126, No. 2, 02.2005, p. 309-315.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glutathione S-transferase hGSTM3 and ageing-associated neurodegeneration
T2 - Relationship to Alzheimer's disease
AU - Tchaikovskaya, Tatyana L.
AU - Fraifeld, Vadim
AU - Urphanishvili, Tinatin
AU - Andorfer, John H.
AU - Davies, Peter
AU - Listowsky, Irving
PY - 2005/2
Y1 - 2005/2
N2 - Glutathione S-transferases (GSTs) are detoxification enzymes that can counter ageing-associated oxidative and chemical stresses. The transcript of a distinct subclass of human GSTs (hGSTM3) was shown by RNA blot analysis to be widely distributed in different regions of adult brain. HPLC profiles indicated that the hGSTM3 subunit was the second most abundant GST subunit in brain. Immunocytochemistry performed with hGSTM3-specific antisera, showed prominent staining of neuritic plaques, neurofibrillary tangles and microglia in sections of hippocampus obtained from patients with Alzheimer's disease. The staining pattern was distinct from that obtained with normal brains. Because hGSTM3 is rich in cysteine residues and readily undergoes S-glutathiolation reactions, deposition of this protein could originate from cross-links produced by oxidative stress.
AB - Glutathione S-transferases (GSTs) are detoxification enzymes that can counter ageing-associated oxidative and chemical stresses. The transcript of a distinct subclass of human GSTs (hGSTM3) was shown by RNA blot analysis to be widely distributed in different regions of adult brain. HPLC profiles indicated that the hGSTM3 subunit was the second most abundant GST subunit in brain. Immunocytochemistry performed with hGSTM3-specific antisera, showed prominent staining of neuritic plaques, neurofibrillary tangles and microglia in sections of hippocampus obtained from patients with Alzheimer's disease. The staining pattern was distinct from that obtained with normal brains. Because hGSTM3 is rich in cysteine residues and readily undergoes S-glutathiolation reactions, deposition of this protein could originate from cross-links produced by oxidative stress.
KW - Ageing
KW - Alzheimer's disease
KW - Brain
KW - Glutathione S-transferases
KW - hGSTM5 subunit
KW - Microglia
UR - http://www.scopus.com/inward/record.url?scp=11144307948&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11144307948&partnerID=8YFLogxK
U2 - 10.1016/j.mad.2004.08.029
DO - 10.1016/j.mad.2004.08.029
M3 - Article
C2 - 15621212
AN - SCOPUS:11144307948
VL - 126
SP - 309
EP - 315
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 2
ER -