Glutamine attenuates tumor necrosis factor-α release and enhances heat shock protein 72 in human peripheral blood mononuclear cells

Paul E. Wischmeyer, Jacob Riehm, Kristen D. Singleton, Hongyu Ren, Mark W. Musch, Madelyn Kahana, Eugene B. Chang

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

OBJECTIVES: Overexpression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) can contribute to multiple organ dysfunction syndrome and septic shock in critically ill patients. We previously found that glutamine (GLN) can attenuate cytokine expression, induce heat shock protein 72 (HSP 72), and protect against endotoxin-induced mortality and organ injury in an in vivo rat model. However, data on the effect of GLN on direct attenuation of cytokine release and HSP 72 expression in human peripheral blood polymorphonuclear cells (PBMCs) is lacking. METHODS: In this study, we assessed the effect of GLN on TNF-α and HSP 72 expression in human PBMCs. After treating with various doses of GLN, human PBMCs were stimulated with lipopolysaccharide (LPS). TNF-α release was analyzed via enzyme-linked immunosorbent assay and HSP 72 via western blot. RESULTS: GLN at doses greater than 4 mM decreased TNF-α release at 4 and 24 h after LPS stimulation. Sublethal heating of PBMCs before LPS also markedly decreased TNF-α after LPS. Doses of GLN greater than 2 to 4 mM led to an increase in HSP 72 expression after LPS. CONCLUSIONS: These results indicate that GLN, which may improve outcomes in critically ill patients, can directly attenuate pro-inflammatory cytokine release in PBMCs. This effect may be related to enhanced HSP 72 expression.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalNutrition
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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HSP72 Heat-Shock Proteins
Glutamine
Blood Cells
Tumor Necrosis Factor-alpha
Lipopolysaccharides
Cytokines
Critical Illness
Multiple Organ Failure
Septic Shock
Endotoxins
Heating
Western Blotting
Enzyme-Linked Immunosorbent Assay
Mortality
Wounds and Injuries

Keywords

  • Amino acid
  • Cytokine
  • Endotoxin
  • Glutamine
  • Inflammation
  • Lipopolysaccharide
  • Sepsis
  • Stress proteins

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery
  • Medicine (miscellaneous)

Cite this

Glutamine attenuates tumor necrosis factor-α release and enhances heat shock protein 72 in human peripheral blood mononuclear cells. / Wischmeyer, Paul E.; Riehm, Jacob; Singleton, Kristen D.; Ren, Hongyu; Musch, Mark W.; Kahana, Madelyn; Chang, Eugene B.

In: Nutrition, Vol. 19, No. 1, 01.01.2003, p. 1-6.

Research output: Contribution to journalArticle

Wischmeyer, Paul E. ; Riehm, Jacob ; Singleton, Kristen D. ; Ren, Hongyu ; Musch, Mark W. ; Kahana, Madelyn ; Chang, Eugene B. / Glutamine attenuates tumor necrosis factor-α release and enhances heat shock protein 72 in human peripheral blood mononuclear cells. In: Nutrition. 2003 ; Vol. 19, No. 1. pp. 1-6.
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