Glutamate receptor gene expression in spinal cord of arthritic rats

Domenico E. Pellegrini-Giampietro, Shaoguang Fan, Brian Ault, Bruce E. Miller, R. Suzanne Zukin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Injury to peripheral tissue leads to hyperalgesia that appears to be partly mediated by functional changes at the level of the spinal cord. Glutamate receptors are thought to play a role in acute and short-term (minutes to hours) spinal cord nociceptive responses and may be involved in prolonged or chronic pain (hours to days). We used in situ hybridization to examine AMPA/kainate (GluR1, GluR2, and GluR3) and NMDA (NR1) receptor gene expression in spinal cord following induction of prolonged inflammation by a unilateral intraarticular injection of lipopolysaccharide (LPS; 10 μg) into the hindpaw. In control rats, GluR1 expression was prominent throughout the layers of the gray matter of the spinal cord. Microscopic examination revealed labeling of neuronal cell somata in all major nuclei. GluR2 was abundant in substantia gelatinosa and motor nuclei; emulsion-dipped sections exhibited intense labeling over densely packed neurons in the superficial laminae of dorsal horn and individual motoneurons of ventral horn. GluR3 and NR1 were expressed at low levels throughout spinal cord gray matter. One day after LPS injection, when joint swelling was maximal, GluR1 expression was bilaterally decreased by 25% in the substantia gelatinosa at the level of the lumbar cord. In contrast, no significant change was apparent in GluR2, GluR3, or NR1 expression in any nucleus of the cord. At 72 hr after injection, when joint diameter approached control values, all four transcripts were expressed at near control levels. These findings provide evidence for a specific decrease in GluR1 expression in the cord in response to joint inflammation.

Original languageEnglish (US)
Pages (from-to)1576-1583
Number of pages8
JournalJournal of Neuroscience
Volume14
Issue number3 II
StatePublished - Mar 1994

Fingerprint

Spondylarthritis
Glutamate Receptors
Spinal Cord
Gene Expression
Substantia Gelatinosa
Joints
Inflammation
Intra-Articular Injections
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Injections
Kainic Acid
Hyperalgesia
Carisoprodol
Motor Neurons
Horns
Emulsions
Chronic Pain
In Situ Hybridization
Lipopolysaccharides
Neurons

Keywords

  • AMPA
  • excitatory amino acid receptors
  • in situ hybridization
  • inflammation
  • kainate
  • NMDA
  • spinal cord plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pellegrini-Giampietro, D. E., Fan, S., Ault, B., Miller, B. E., & Zukin, R. S. (1994). Glutamate receptor gene expression in spinal cord of arthritic rats. Journal of Neuroscience, 14(3 II), 1576-1583.

Glutamate receptor gene expression in spinal cord of arthritic rats. / Pellegrini-Giampietro, Domenico E.; Fan, Shaoguang; Ault, Brian; Miller, Bruce E.; Zukin, R. Suzanne.

In: Journal of Neuroscience, Vol. 14, No. 3 II, 03.1994, p. 1576-1583.

Research output: Contribution to journalArticle

Pellegrini-Giampietro, DE, Fan, S, Ault, B, Miller, BE & Zukin, RS 1994, 'Glutamate receptor gene expression in spinal cord of arthritic rats', Journal of Neuroscience, vol. 14, no. 3 II, pp. 1576-1583.
Pellegrini-Giampietro DE, Fan S, Ault B, Miller BE, Zukin RS. Glutamate receptor gene expression in spinal cord of arthritic rats. Journal of Neuroscience. 1994 Mar;14(3 II):1576-1583.
Pellegrini-Giampietro, Domenico E. ; Fan, Shaoguang ; Ault, Brian ; Miller, Bruce E. ; Zukin, R. Suzanne. / Glutamate receptor gene expression in spinal cord of arthritic rats. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 3 II. pp. 1576-1583.
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