Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells

Background: Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysismembrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG)has an identical effect.Methods: Replicative aging of MCs was studied during 10 passages performed every three days incells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30mmol/L. Changes in population doubling time and β-galactosidase activity were used as an index ofaging and compared with other cellular parameters.Results: Repeated passages of MC cause their aging, as reflected by prolongation of the populationdoubling time, increased β-galactosidase activity, oxidative stress and release of cytokines. Healing ofinjured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC,whereas NAG does not cause this effect.Conclusions: Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.