Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells

Marta Ciszewicz, George Wu, Paul Tam, Alicja Polubinska, Andrzej Brȩborowicz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysismembrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG)has an identical effect.Methods: Replicative aging of MCs was studied during 10 passages performed every three days incells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30mmol/L. Changes in population doubling time and β-galactosidase activity were used as an index ofaging and compared with other cellular parameters.Results: Repeated passages of MC cause their aging, as reflected by prolongation of the populationdoubling time, increased β-galactosidase activity, oxidative stress and release of cytokines. Healing ofinjured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC,whereas NAG does not cause this effect.Conclusions: Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.

Original languageEnglish (US)
Pages (from-to)489-494
Number of pages6
JournalInternational Journal of Artificial Organs
Volume34
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Fingerprint

Acetylglucosamine
Cell Aging
Glucose
Aging of materials
Galactosidases
Oxidative stress
Dialysis
Oxidative Stress
Cytokines
Monolayers
In Vitro Techniques
Fluids
Population

Keywords

  • Aging
  • Glucose
  • Healing
  • Mesothelium
  • N-acetylglucosamine

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells. / Ciszewicz, Marta; Wu, George; Tam, Paul; Polubinska, Alicja; Brȩborowicz, Andrzej.

In: International Journal of Artificial Organs, Vol. 34, No. 6, 06.2011, p. 489-494.

Research output: Contribution to journalArticle

Ciszewicz, Marta ; Wu, George ; Tam, Paul ; Polubinska, Alicja ; Brȩborowicz, Andrzej. / Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells. In: International Journal of Artificial Organs. 2011 ; Vol. 34, No. 6. pp. 489-494.
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