Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells

Marta Ciszewicz, George Wu, Paul Tam, Alicja Polubinska, Andrzej Brȩborowicz

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysismembrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG)has an identical effect.Methods: Replicative aging of MCs was studied during 10 passages performed every three days incells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30mmol/L. Changes in population doubling time and β-galactosidase activity were used as an index ofaging and compared with other cellular parameters.Results: Repeated passages of MC cause their aging, as reflected by prolongation of the populationdoubling time, increased β-galactosidase activity, oxidative stress and release of cytokines. Healing ofinjured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC,whereas NAG does not cause this effect.Conclusions: Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.

Original languageEnglish (US)
Pages (from-to)489-494
Number of pages6
JournalInternational Journal of Artificial Organs
Volume34
Issue number6
DOIs
StatePublished - Jun 1 2011

Keywords

  • Aging
  • Glucose
  • Healing
  • Mesothelium
  • N-acetylglucosamine

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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