Glomerular basement membrane metabolism in the diabetic rat. In vivo studies

M. Brownlee, R. G. Spiro

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The effect of diabetes on the metabolism of the renal glomerular basement membrane has been studied in the rat with the aid of injected tracer doses of tritiated proline. At various times after administration of the labeled amino acid, the specific radioactivities of the proline and hydroxyproline of the basement membranes from alloxan diabetic rats were determined and compared with those of age-matched normal rats. In both normal and diabetic animals the incorporation of radioactivity into the basement membrane was slow and, after a maximum was reached, an extended period of almost constant specific activity of proline and hydroxyproline was observed. The diabetic basement membrane, however, differed from the normal by attaining specific activities of the amino acids which were about twice as high as normal (P < 0.001 at 42 hr after injection of radioisotope). Although the proline concentration of serum and renal cortical fluid was the same in normal and diabetic rats, there were substantial differences in the specific activity of this precursor amino acid in these pools that had to be taken into account to compare the 2 types of animals. The results of the present study are consistent with an accelerated rate of glomerular basement membrane polypeptide synthesis and proline.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalDiabetes
Volume28
Issue number2
StatePublished - 1979
Externally publishedYes

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Glomerular Basement Membrane
Proline
Basement Membrane
Hydroxyproline
Amino Acids
Radioactivity
Kidney
Alloxan
Radioisotopes
Peptides
Injections
Serum

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Brownlee, M., & Spiro, R. G. (1979). Glomerular basement membrane metabolism in the diabetic rat. In vivo studies. Diabetes, 28(2), 121-125.

Glomerular basement membrane metabolism in the diabetic rat. In vivo studies. / Brownlee, M.; Spiro, R. G.

In: Diabetes, Vol. 28, No. 2, 1979, p. 121-125.

Research output: Contribution to journalArticle

Brownlee, M & Spiro, RG 1979, 'Glomerular basement membrane metabolism in the diabetic rat. In vivo studies', Diabetes, vol. 28, no. 2, pp. 121-125.
Brownlee, M. ; Spiro, R. G. / Glomerular basement membrane metabolism in the diabetic rat. In vivo studies. In: Diabetes. 1979 ; Vol. 28, No. 2. pp. 121-125.
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