Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients

Hui chan He, Zhao dong Han, Qi shan Dai, Xiao hui Ling, Xin Fu, Zhuo yuan Lin, Ye han Deng, Guo qiang Qin, Chao Cai, Jia hong Chen, Fu neng Jiang, Xingyin Liu, Wei de Zhong

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40 Citations (Scopus)

Abstract

Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.Results: A total of 28 miRNAs (fold change ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

Original languageEnglish (US)
Article number757
JournalBMC Genomics
Volume14
Issue number1
DOIs
StatePublished - Nov 5 2013

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MicroRNAs
Prostatic Neoplasms
Population
Messenger RNA
Microarray Analysis
Computational Biology
Genes
Recurrence
Polymerase Chain Reaction

Keywords

  • miR-374b
  • miRNA expression profile
  • mRNA expression profile
  • Prostate cancer

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

He, H. C., Han, Z. D., Dai, Q. S., Ling, X. H., Fu, X., Lin, Z. Y., ... Zhong, W. D. (2013). Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients. BMC Genomics, 14(1), [757]. https://doi.org/10.1186/1471-2164-14-757

Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients. / He, Hui chan; Han, Zhao dong; Dai, Qi shan; Ling, Xiao hui; Fu, Xin; Lin, Zhuo yuan; Deng, Ye han; Qin, Guo qiang; Cai, Chao; Chen, Jia hong; Jiang, Fu neng; Liu, Xingyin; Zhong, Wei de.

In: BMC Genomics, Vol. 14, No. 1, 757, 05.11.2013.

Research output: Contribution to journalArticle

He, HC, Han, ZD, Dai, QS, Ling, XH, Fu, X, Lin, ZY, Deng, YH, Qin, GQ, Cai, C, Chen, JH, Jiang, FN, Liu, X & Zhong, WD 2013, 'Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients', BMC Genomics, vol. 14, no. 1, 757. https://doi.org/10.1186/1471-2164-14-757
He, Hui chan ; Han, Zhao dong ; Dai, Qi shan ; Ling, Xiao hui ; Fu, Xin ; Lin, Zhuo yuan ; Deng, Ye han ; Qin, Guo qiang ; Cai, Chao ; Chen, Jia hong ; Jiang, Fu neng ; Liu, Xingyin ; Zhong, Wei de. / Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients. In: BMC Genomics. 2013 ; Vol. 14, No. 1.
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abstract = "Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60{\%} of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.Results: A total of 28 miRNAs (fold change ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.",
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AU - Fu, Xin

AU - Lin, Zhuo yuan

AU - Deng, Ye han

AU - Qin, Guo qiang

AU - Cai, Chao

AU - Chen, Jia hong

AU - Jiang, Fu neng

AU - Liu, Xingyin

AU - Zhong, Wei de

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N2 - Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.Results: A total of 28 miRNAs (fold change ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

AB - Background: Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.Results: A total of 28 miRNAs (fold change ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.Conclusions: Our findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.

KW - miR-374b

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