GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice

Fayyaz Ahmed, Jeffrey Wyckoff, Elaine Y. Lin, Weigang Wang, Yarong Wang, Lothar Hennighausen, Jun ichi Miyazaki, Joan Jones, Jeffrey W. Pollard, John S. Condeelis, Jeffrey E. Segall

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

To examine the behavior of tumor cells in tumors developing directly from mammary tissue in transgenic models, we have evaluated transgenic mice expressing green fluorescent protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific. These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution.

Original languageEnglish (US)
Pages (from-to)7166-7169
Number of pages4
JournalCancer research
Volume62
Issue number24
StatePublished - Dec 15 2002

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Ahmed, F., Wyckoff, J., Lin, E. Y., Wang, W., Wang, Y., Hennighausen, L., Miyazaki, J. I., Jones, J., Pollard, J. W., Condeelis, J. S., & Segall, J. E. (2002). GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice. Cancer research, 62(24), 7166-7169.