Get it together: How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo

Odelya Hartung, Florence L. Marlow

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

In zebrafish, the sequential processes of oocyte maturation, egg fertilization, and early embryonic development take place while the genome is transcriptionally silent. Proper development and patterning of the oocyte and embryo requires that the numerous gene products (RNAs and proteins) that regulate oocyte and early embryonic development are produced prior to genome inactivation and are preserved and utilized in the time and place where they are needed. During this period the oocyte and eventual embryo relies on posttranscriptional and posttranslational mechanisms. Prominent regulators are the various RNA-binding proteins (RNAbps) that assemble their RNA targets into ribonucleoprotein (RNP) granules where they are localized, protected, translated or degraded. In oocytes and embryos, these RNP granules are enriched in an electron-dense portion of cytoplasm termed germ plasm, so-called because its contents include the molecules that induce formation of future germ cells. In this chapter, we will review the roles of RNAbps and posttranscriptional regulation in the germ plasm of developing zebrafish oocytes and embryonic primordial germ cells.

Original languageEnglish (US)
Title of host publicationZebrafish: Topics in Reproduction, Toxicology and Development
PublisherNova Science Publishers, Inc.
Pages65-106
Number of pages42
ISBN (Print)9781631175695, 9781631175589
StatePublished - Apr 1 2014

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
Oocytes
embryo (animal)
germplasm
oocytes
Embryonic Structures
ribonucleoproteins
Ribonucleoproteins
Zebrafish
Danio rerio
Embryonic Development
granules
germ cells
embryogenesis
Genome
RNA
genome
Fertilization
Germ Cells

Keywords

  • Balbiani body
  • Bucky ball
  • Dazl
  • Dead End
  • Germ granule
  • Germ plasm
  • Nanos3
  • Oocyte
  • Primordial Germ Cell
  • RNA binding protein
  • Vasa

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Hartung, O., & Marlow, F. L. (2014). Get it together: How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo. In Zebrafish: Topics in Reproduction, Toxicology and Development (pp. 65-106). Nova Science Publishers, Inc..

Get it together : How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo. / Hartung, Odelya; Marlow, Florence L.

Zebrafish: Topics in Reproduction, Toxicology and Development. Nova Science Publishers, Inc., 2014. p. 65-106.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hartung, O & Marlow, FL 2014, Get it together: How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo. in Zebrafish: Topics in Reproduction, Toxicology and Development. Nova Science Publishers, Inc., pp. 65-106.
Hartung O, Marlow FL. Get it together: How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo. In Zebrafish: Topics in Reproduction, Toxicology and Development. Nova Science Publishers, Inc. 2014. p. 65-106
Hartung, Odelya ; Marlow, Florence L. / Get it together : How RNA-binding proteins assemble and regulate germ plasm in the oocyte and embryo. Zebrafish: Topics in Reproduction, Toxicology and Development. Nova Science Publishers, Inc., 2014. pp. 65-106
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