Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi

Donna E. Akiyoshi, Hilary G. Morrison, Shi Lei, Xiaochuan Feng, Quanshun Zhang, Nicolas Corradi, Harriet Mayanja, James K. Tumwine, Patrick J. Keeling, Louis M. Weiss, Saul Tzipori

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Enterocytozoon bieneusi is the most common microsporidian associated with human disease, particularly in the immunocompromised population. In the setting of HIV infection, it is associated with diarrhea and wasting syndrome. Like all microsporidia, E. bieneusi is an obligate, intracellular parasite, but unlike others, it is in direct contact with the host cell cytoplasm. Studies of E. bieneusi have been greatly limited due to the absence of genomic data and lack of a robust cultivation system. Here, we present the first large-scale genomic dataset for E. bieneusi. Approximately 3.86 Mb of unique sequence was generated by paired end Sanger sequencing, representing about 64% of the estimated 6 Mb genome. A total of 3,804 genes were identified in E. bieneusi, of which 1,702 encode proteins with assigned functions. Of these, 653 are homologs of Encephalitozoon cuniculi proteins. Only one E. bieneusi protein with assigned function had no E. cuniculi homolog. The shared proteins were, in general, evenly distributed among the functional categories, with the exception of a dearth of genes encoding proteins associated with pathways for fatty acid and core carbon metabolism. Short intergenic regions, high gene density, and shortened protein-coding sequences were observed in the E. bieneusi genome, all traits consistent with genomic compaction. Our findings suggest that E. bieneusi is a likely model for extreme genome reduction and host dependence.

Original languageEnglish (US)
Article numbere1000261
JournalPLoS Pathogens
Volume5
Issue number1
DOIs
StatePublished - Jan 2009

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Enterocytozoon
Encephalitozoon cuniculi
Proteins
Genome
Microsporidia
Wasting Syndrome
Intergenic DNA
Surveys and Questionnaires
Genes
HIV Infections
Diarrhea
Cytoplasm
Parasites
Fatty Acids
Carbon

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Akiyoshi, D. E., Morrison, H. G., Lei, S., Feng, X., Zhang, Q., Corradi, N., ... Tzipori, S. (2009). Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. PLoS Pathogens, 5(1), [e1000261]. https://doi.org/10.1371/journal.ppat.1000261

Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. / Akiyoshi, Donna E.; Morrison, Hilary G.; Lei, Shi; Feng, Xiaochuan; Zhang, Quanshun; Corradi, Nicolas; Mayanja, Harriet; Tumwine, James K.; Keeling, Patrick J.; Weiss, Louis M.; Tzipori, Saul.

In: PLoS Pathogens, Vol. 5, No. 1, e1000261, 01.2009.

Research output: Contribution to journalArticle

Akiyoshi, DE, Morrison, HG, Lei, S, Feng, X, Zhang, Q, Corradi, N, Mayanja, H, Tumwine, JK, Keeling, PJ, Weiss, LM & Tzipori, S 2009, 'Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi', PLoS Pathogens, vol. 5, no. 1, e1000261. https://doi.org/10.1371/journal.ppat.1000261
Akiyoshi, Donna E. ; Morrison, Hilary G. ; Lei, Shi ; Feng, Xiaochuan ; Zhang, Quanshun ; Corradi, Nicolas ; Mayanja, Harriet ; Tumwine, James K. ; Keeling, Patrick J. ; Weiss, Louis M. ; Tzipori, Saul. / Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. In: PLoS Pathogens. 2009 ; Vol. 5, No. 1.
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