Genomes of marine cyanopodoviruses reveal multiple origins of diversity

S. J. Labrie, K. Frois-Moniz, M. S. Osburne, Libusha Kelly, S. E. Roggensack, M. B. Sullivan, G. Gearin, Q. Zeng, M. Fitzgerald, M. R. Henn, S. W. Chisholm

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The marine cyanobacteria Prochlorococcus and Synechococcus are highly abundant in the global oceans, as are the cyanophage with which they co-evolve. While genomic analyses have been relatively extensive for cyanomyoviruses, only three cyanopodoviruses isolated on marine cyanobacteria have been sequenced. Here we present nine new cyanopodovirus genomes, and analyse them in the context of the broader group. The genomes range from 42.2 to 47.7kb, with G+C contents consistent with those of their hosts. They share 12 core genes, and the pan-genome is not close to being fully sampled. The genomes contain three variable island regions, with the most hypervariable genes concentrated at one end of the genome. Concatenated core-gene phylogeny clusters all but one of the phage into three distinct groups (MPP-A and two discrete clades within MPP-B). The outlier, P-RSP2, has the smallest genome and lacks RNA polymerase, a hallmark of the Autographivirinae subfamily. The phage in group MPP-B contain photosynthesis and carbon metabolism associated genes, while group MPP-A and the outlier P-RSP2 do not, suggesting different constraints on their lytic cycles. Four of the phage encode integrases and three have a host integration signature. Metagenomic analyses reveal that cyanopodoviruses may be more abundant in the oceans than previously thought.

Original languageEnglish (US)
Pages (from-to)1356-1376
Number of pages21
JournalEnvironmental Microbiology
Volume15
Issue number5
DOIs
StatePublished - May 2013
Externally publishedYes

Fingerprint

genome
Genome
bacteriophages
Bacteriophages
gene
Cyanobacteria
outlier
Oceans and Seas
cyanobacterium
Prochlorococcus
genes
oceans
Genes
Synechococcus
Integrases
Metagenomics
Base Composition
Photosynthesis
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Labrie, S. J., Frois-Moniz, K., Osburne, M. S., Kelly, L., Roggensack, S. E., Sullivan, M. B., ... Chisholm, S. W. (2013). Genomes of marine cyanopodoviruses reveal multiple origins of diversity. Environmental Microbiology, 15(5), 1356-1376. https://doi.org/10.1111/1462-2920.12053

Genomes of marine cyanopodoviruses reveal multiple origins of diversity. / Labrie, S. J.; Frois-Moniz, K.; Osburne, M. S.; Kelly, Libusha; Roggensack, S. E.; Sullivan, M. B.; Gearin, G.; Zeng, Q.; Fitzgerald, M.; Henn, M. R.; Chisholm, S. W.

In: Environmental Microbiology, Vol. 15, No. 5, 05.2013, p. 1356-1376.

Research output: Contribution to journalArticle

Labrie, SJ, Frois-Moniz, K, Osburne, MS, Kelly, L, Roggensack, SE, Sullivan, MB, Gearin, G, Zeng, Q, Fitzgerald, M, Henn, MR & Chisholm, SW 2013, 'Genomes of marine cyanopodoviruses reveal multiple origins of diversity', Environmental Microbiology, vol. 15, no. 5, pp. 1356-1376. https://doi.org/10.1111/1462-2920.12053
Labrie SJ, Frois-Moniz K, Osburne MS, Kelly L, Roggensack SE, Sullivan MB et al. Genomes of marine cyanopodoviruses reveal multiple origins of diversity. Environmental Microbiology. 2013 May;15(5):1356-1376. https://doi.org/10.1111/1462-2920.12053
Labrie, S. J. ; Frois-Moniz, K. ; Osburne, M. S. ; Kelly, Libusha ; Roggensack, S. E. ; Sullivan, M. B. ; Gearin, G. ; Zeng, Q. ; Fitzgerald, M. ; Henn, M. R. ; Chisholm, S. W. / Genomes of marine cyanopodoviruses reveal multiple origins of diversity. In: Environmental Microbiology. 2013 ; Vol. 15, No. 5. pp. 1356-1376.
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