Genome-wide, Single-Cell DNA Methylomics Reveals Increased Non-CpG Methylation during Human Oocyte Maturation

Bo Yu, Xiao Dong, Silvia Gravina, Önder Kartal, Timothy Schimmel, Jacques Cohen, Drew Tortoriello, Raifa Zody, R. David Hawkins, Jan Vijg

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The establishment of DNA methylation patterns in oocytes is a highly dynamic process marking gene-regulatory events during fertilization, embryonic development, and adulthood. However, after epigenetic reprogramming in primordial germ cells, how and when DNA methylation is re-established in developing human oocytes remains to be characterized. Here, using single-cell whole-genome bisulfite sequencing, we describe DNA methylation patterns in three different maturation stages of human oocytes. We found that while broad-scale patterns of CpG methylation have been largely established by the immature germinal vesicle stage, localized changes continue into later development. Non-CpG methylation, on the other hand, undergoes a large-scale, generalized remodeling through the final stage of maturation, with the net overall result being the accumulation of methylation as oocytes mature. The role of the genome-wide, non-CpG methylation remodeling in the final stage of oocyte maturation deserves further investigation. CpG methylation is established globally in adult immature oocytes and remains stable during maturation. Non-CpG methylation undergoes a genome-wide, generalized remodeling through the final stage of maturation, with the net overall result being accumulation of methylation as oocytes mature.

Original languageEnglish (US)
JournalStem Cell Reports
DOIs
StateAccepted/In press - Jan 23 2017

Fingerprint

Methylation
Oocytes
Genes
Genome
DNA
DNA Methylation
Regulator Genes
Fertilization
Epigenomics
Germ Cells
Embryonic Development
Cells

Keywords

  • DNA methylome
  • Epigenome
  • Human oocyte
  • In vitro maturation
  • Non-CpG methylation
  • Oocyte maturation
  • Single cell

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Genome-wide, Single-Cell DNA Methylomics Reveals Increased Non-CpG Methylation during Human Oocyte Maturation. / Yu, Bo; Dong, Xiao; Gravina, Silvia; Kartal, Önder; Schimmel, Timothy; Cohen, Jacques; Tortoriello, Drew; Zody, Raifa; Hawkins, R. David; Vijg, Jan.

In: Stem Cell Reports, 23.01.2017.

Research output: Contribution to journalArticle

Yu, Bo ; Dong, Xiao ; Gravina, Silvia ; Kartal, Önder ; Schimmel, Timothy ; Cohen, Jacques ; Tortoriello, Drew ; Zody, Raifa ; Hawkins, R. David ; Vijg, Jan. / Genome-wide, Single-Cell DNA Methylomics Reveals Increased Non-CpG Methylation during Human Oocyte Maturation. In: Stem Cell Reports. 2017.
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