Genome-wide remodeling of the epigenetic landscape during myogenic differentiation

Patrik K.M. Asp, Roy Blum, Vasupradha Vethantham, Fabio Parisi, Mariann Micsinai, Jemmie Cheng, Christopher Bowman, Yuval Kluger, Brian David Dynlacht

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

We have examined changes in the chromatin landscape during muscle differentiation by mapping the genome-wide location of ten key histone marks and transcription factors in mouse myoblasts and terminally differentiated myotubes, providing an exceptionally rich dataset that has enabled discovery of key epigenetic changes underlying myogenesis. Using this compendium, we focused on a well-known repressive mark, histone H3 lysine 27 trimethylation, and identified novel regulatory elements flanking the myogenin gene that function as a key differentiation-dependent switch during myogenesis. Next, we examined the role of Polycomb-mediated H3K27 methylation in gene repression by systematically ablating components of both PRC1 and PRC2 complexes. Surprisingly, we found mechanistic differences between transient and permanent repression of muscle differentiation and lineage commitment genes and observed that the loss of PRC1 and PRC2 components produced opposing differentiation defects. These phenotypes illustrate striking differences as compared to embryonic stem cell differentiation and suggest that PRC1 and PRC2 do not operate sequentially in muscle cells. Our studies of PRC1 occupancy also suggested a "fail-safe" mechanism, whereby PRC1/Bmi1 concentrates at genes specifying nonmuscle lineages, helping to retain H3K27me3 in the face of declining Ezh2-mediated methyltransferase activity in differentiated cells.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number22
DOIs
StatePublished - May 31 2011

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Epigenomics
Genome
Muscle Development
Genes
Histone Code
Myogenin
Muscles
Myoblasts
Chromosome Mapping
Skeletal Muscle Fibers
Methyltransferases
Embryonic Stem Cells
Histones
Muscle Cells
Methylation
Lysine
Chromatin
Cell Differentiation
Transcription Factors
Phenotype

Keywords

  • Chip-Seq
  • Chromatin modifications
  • Muscle development
  • Transcriptional regulation

ASJC Scopus subject areas

  • General

Cite this

Genome-wide remodeling of the epigenetic landscape during myogenic differentiation. / Asp, Patrik K.M.; Blum, Roy; Vethantham, Vasupradha; Parisi, Fabio; Micsinai, Mariann; Cheng, Jemmie; Bowman, Christopher; Kluger, Yuval; Dynlacht, Brian David.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 22, 31.05.2011.

Research output: Contribution to journalArticle

Asp, Patrik K.M. ; Blum, Roy ; Vethantham, Vasupradha ; Parisi, Fabio ; Micsinai, Mariann ; Cheng, Jemmie ; Bowman, Christopher ; Kluger, Yuval ; Dynlacht, Brian David. / Genome-wide remodeling of the epigenetic landscape during myogenic differentiation. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 22.
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