Genome wide methylome alterations in lung cancer

Nandita Mullapudi, Bin Ye, Masako Suzuki, Melissa Fazzari, Weiguo Han, Miao K. Shi, Gaby Marquardt, Juan Lin, Tao Wang, Steven Keller, Changcheng Zhu, Joseph D. Locker, Simon D. Spivack

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Aberrant cytosine 5-methylation underlies many deregulated elements of cancer. Among paired non-small cell lung cancers (NSCLC), we sought to profile DNA 5-methyl-cytosine features which may underlie genome-wide deregulation. In one of the more dense interrogations of the methylome, we sampled 1.2 million CpG sites from twenty-four NSCLC tumor (T)-non-Tumor (NT) pairs using a methylation-sensitive restriction enzyme-based HELPmicroarray assay. We found 225,350 differentially methylated (DM) sites in adenocarcinomas versus adjacent non-Tumor tissue that vary in frequency across genomic compartment, particularly notable in gene bodies (GB; p

Original languageEnglish (US)
Article numbere0143826
JournalPLoS One
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2015

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mullapudi, N., Ye, B., Suzuki, M., Fazzari, M., Han, W., Shi, M. K., Marquardt, G., Lin, J., Wang, T., Keller, S., Zhu, C., Locker, J. D., & Spivack, S. D. (2015). Genome wide methylome alterations in lung cancer. PLoS One, 10(12), [e0143826]. https://doi.org/10.1371/journal.pone.0143826