Genome-wide DNA Methylation Analysis Using Massively Parallel Sequencing Technologies

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

"Epigenetics" refers to a heritable change in transcriptional status without alteration in the primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control and plays a crucial role in normal development, as well as in pathological conditions. DNA methylation is one of the best known and well-studied epigenetic modifications. Genome-wide DNA methylation profiling has become recognized as a biologically and clinically important epigenomic assay. In this review, we discuss the strengths and weaknesses of the protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS) techniques. We will also describe recently discovered DNA modifications, and the protocols to detect these modifications.

Original languageEnglish (US)
Pages (from-to)70-77
Number of pages8
JournalSeminars in hematology
Volume50
Issue number1
DOIs
StatePublished - Jan 1 2013

ASJC Scopus subject areas

  • Hematology

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