Genome-wide DNA Methylation Analysis Using Massively Parallel Sequencing Technologies

Masako Suzuki; John M. Greally

doi:10.1053/j.seminhematol.2013.01.002

Genome-wide DNA Methylation Analysis Using Massively Parallel Sequencing Technologies

Masako Suzuki, John M. Greally

Genetics

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

"Epigenetics" refers to a heritable change in transcriptional status without alteration in the primary nucleotide sequence. Epigenetics provides an extra layer of transcriptional control and plays a crucial role in normal development, as well as in pathological conditions. DNA methylation is one of the best known and well-studied epigenetic modifications. Genome-wide DNA methylation profiling has become recognized as a biologically and clinically important epigenomic assay. In this review, we discuss the strengths and weaknesses of the protocols for genome-wide DNA methylation profiling using massively parallel sequencing (MPS) techniques. We will also describe recently discovered DNA modifications, and the protocols to detect these modifications.

Original language	English (US)
Pages (from-to)	70-77
Number of pages	8
Journal	Seminars in hematology
Volume	50
Issue number	1
DOIs	https://doi.org/10.1053/j.seminhematol.2013.01.002
State	Published - Jan 2013

ASJC Scopus subject areas

Hematology

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10.1053/j.seminhematol.2013.01.002

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Genome-wide DNA Methylation Analysis Using Massively Parallel Sequencing Technologies

Abstract

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