Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

Kohta Ikegami; Misa Iwatani; Masako Suzuki; Makoto Tachibana; Yoichi Shinkai; Satoshi Tanaka; John M. Greally; Shintaro Yagi; Naka Hattori; Kunio Shiota

doi:10.1111/j.1365-2443.2006.01029.x

Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

Kohta Ikegami, Misa Iwatani, Masako Suzuki, Makoto Tachibana, Yoichi Shinkai, Satoshi Tanaka, John M. Greally, Shintaro Yagi, Naka Hattori, Kunio Shiota

Genetics

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

In the mammalian genome, numerous CpG-rich loci define tissue-dependent and differentially methylated regions (T-DMRs). Euchromatin from different cell types differs in terms of its tissue-specific DNA methylation profile as defined by these T-DMRs. G9a is a euchromatin-localized histone methyltransferase (HMT) and catalyzes methylation of histone H3 at lysines 9 and 27 (H3-K9 and -K27). To test whether HMT activity influences euchromatic cytosine methylation, we analyzed the DNA methylation status of approximately 2000 CpG-rich loci, which are predicted in silico, in G9a^-/- embryonic stem cells by restriction landmark genomic scanning (RLGS). While the RLGS profile of wild-type cells contained about 1300 spots, 32 new spots indicating DNA demethylation were seen in the profile of G9a^-/- cells. Virtual-image RLGS (Vi-RLGS) allowed us to identify the genomic source of ten of these spots. These were confirmed to be cytosine demethylated, not just at the Not I site detected by the RLGS but extending over several kilobase pairs in cis. Chromatin immunoprecipitation (ChIP) confirmed these locito be targets of G9a, with decreased H3-K9 and/or -K27 dimethylation in the G9a^-/- cells. These data indicate that G9a site-selectively contributes to DNA methylation.

Original language	English (US)
Pages (from-to)	1-11
Number of pages	11
Journal	Genes to Cells
Volume	12
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2443.2006.01029.x
State	Published - Jan 2007

ASJC Scopus subject areas

Genetics
Cell Biology

Access to Document

10.1111/j.1365-2443.2006.01029.x

Cite this

Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells. / Ikegami, Kohta; Iwatani, Misa; Suzuki, Masako; Tachibana, Makoto; Shinkai, Yoichi; Tanaka, Satoshi; Greally, John M.; Yagi, Shintaro; Hattori, Naka; Shiota, Kunio.

In: Genes to Cells, Vol. 12, No. 1, 01.2007, p. 1-11.

Research output: Contribution to journal › Article › peer-review

@article{caebfc5e91cc45459623fc1572ee4f89,

title = "Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells",

abstract = "In the mammalian genome, numerous CpG-rich loci define tissue-dependent and differentially methylated regions (T-DMRs). Euchromatin from different cell types differs in terms of its tissue-specific DNA methylation profile as defined by these T-DMRs. G9a is a euchromatin-localized histone methyltransferase (HMT) and catalyzes methylation of histone H3 at lysines 9 and 27 (H3-K9 and -K27). To test whether HMT activity influences euchromatic cytosine methylation, we analyzed the DNA methylation status of approximately 2000 CpG-rich loci, which are predicted in silico, in G9a-/- embryonic stem cells by restriction landmark genomic scanning (RLGS). While the RLGS profile of wild-type cells contained about 1300 spots, 32 new spots indicating DNA demethylation were seen in the profile of G9a-/- cells. Virtual-image RLGS (Vi-RLGS) allowed us to identify the genomic source of ten of these spots. These were confirmed to be cytosine demethylated, not just at the Not I site detected by the RLGS but extending over several kilobase pairs in cis. Chromatin immunoprecipitation (ChIP) confirmed these locito be targets of G9a, with decreased H3-K9 and/or -K27 dimethylation in the G9a-/- cells. These data indicate that G9a site-selectively contributes to DNA methylation.",

author = "Kohta Ikegami and Misa Iwatani and Masako Suzuki and Makoto Tachibana and Yoichi Shinkai and Satoshi Tanaka and Greally, {John M.} and Shintaro Yagi and Naka Hattori and Kunio Shiota",

year = "2007",

month = jan,

doi = "10.1111/j.1365-2443.2006.01029.x",

language = "English (US)",

volume = "12",

pages = "1--11",

journal = "Genes to Cells",

issn = "1356-9597",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

AU - Ikegami, Kohta

AU - Iwatani, Misa

AU - Suzuki, Masako

AU - Tachibana, Makoto

AU - Shinkai, Yoichi

AU - Tanaka, Satoshi

AU - Greally, John M.

AU - Yagi, Shintaro

AU - Hattori, Naka

AU - Shiota, Kunio

PY - 2007/1

Y1 - 2007/1

N2 - In the mammalian genome, numerous CpG-rich loci define tissue-dependent and differentially methylated regions (T-DMRs). Euchromatin from different cell types differs in terms of its tissue-specific DNA methylation profile as defined by these T-DMRs. G9a is a euchromatin-localized histone methyltransferase (HMT) and catalyzes methylation of histone H3 at lysines 9 and 27 (H3-K9 and -K27). To test whether HMT activity influences euchromatic cytosine methylation, we analyzed the DNA methylation status of approximately 2000 CpG-rich loci, which are predicted in silico, in G9a-/- embryonic stem cells by restriction landmark genomic scanning (RLGS). While the RLGS profile of wild-type cells contained about 1300 spots, 32 new spots indicating DNA demethylation were seen in the profile of G9a-/- cells. Virtual-image RLGS (Vi-RLGS) allowed us to identify the genomic source of ten of these spots. These were confirmed to be cytosine demethylated, not just at the Not I site detected by the RLGS but extending over several kilobase pairs in cis. Chromatin immunoprecipitation (ChIP) confirmed these locito be targets of G9a, with decreased H3-K9 and/or -K27 dimethylation in the G9a-/- cells. These data indicate that G9a site-selectively contributes to DNA methylation.

AB - In the mammalian genome, numerous CpG-rich loci define tissue-dependent and differentially methylated regions (T-DMRs). Euchromatin from different cell types differs in terms of its tissue-specific DNA methylation profile as defined by these T-DMRs. G9a is a euchromatin-localized histone methyltransferase (HMT) and catalyzes methylation of histone H3 at lysines 9 and 27 (H3-K9 and -K27). To test whether HMT activity influences euchromatic cytosine methylation, we analyzed the DNA methylation status of approximately 2000 CpG-rich loci, which are predicted in silico, in G9a-/- embryonic stem cells by restriction landmark genomic scanning (RLGS). While the RLGS profile of wild-type cells contained about 1300 spots, 32 new spots indicating DNA demethylation were seen in the profile of G9a-/- cells. Virtual-image RLGS (Vi-RLGS) allowed us to identify the genomic source of ten of these spots. These were confirmed to be cytosine demethylated, not just at the Not I site detected by the RLGS but extending over several kilobase pairs in cis. Chromatin immunoprecipitation (ChIP) confirmed these locito be targets of G9a, with decreased H3-K9 and/or -K27 dimethylation in the G9a-/- cells. These data indicate that G9a site-selectively contributes to DNA methylation.

UR - http://www.scopus.com/inward/record.url?scp=33845781998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845781998&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2443.2006.01029.x

DO - 10.1111/j.1365-2443.2006.01029.x

M3 - Article

C2 - 17212651

AN - SCOPUS:33845781998

VL - 12

SP - 1

EP - 11

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 1

ER -

Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this