Genome scanning of breast cancers by two-dimensional DNA typing

A. M. Verwest, W. J F de Leeuw, A. C. Molijn, T. I. Andersen, A. L. Börresen, E. Mullaart, A. G. Uitterlinden, Jan Vijg

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time. Here we demonstrate the potency of this method for total genome scanning of the tumour genome by analysing a small series of breast cancers. Comparison of tumour and normal DNA from ten breast cancer patients, using two-dimensional DNA typing with four core probes, revealed a considerable number of genomic alterations. In contrast, with Southern blot analysis only a few alterations were observed using the same probes. Most of the changes observed (74%) were deletions (absence of spots in the tumour) while 20% corresponded to amplifications (spots of higher intensity in the tumour) and 5% were new spots (gains). About 10% of the genomic changes detected appeared to occur in the tumours of more than one patient.

Original languageEnglish (US)
Pages (from-to)84-92
Number of pages9
JournalBritish Journal of Cancer
Volume69
Issue number1
StatePublished - Jan 1994
Externally publishedYes

Fingerprint

DNA Fingerprinting
Genome
Breast Neoplasms
Neoplasms
Gels
Minisatellite Repeats
Southern Blotting
Microsatellite Repeats
DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Verwest, A. M., de Leeuw, W. J. F., Molijn, A. C., Andersen, T. I., Börresen, A. L., Mullaart, E., ... Vijg, J. (1994). Genome scanning of breast cancers by two-dimensional DNA typing. British Journal of Cancer, 69(1), 84-92.

Genome scanning of breast cancers by two-dimensional DNA typing. / Verwest, A. M.; de Leeuw, W. J F; Molijn, A. C.; Andersen, T. I.; Börresen, A. L.; Mullaart, E.; Uitterlinden, A. G.; Vijg, Jan.

In: British Journal of Cancer, Vol. 69, No. 1, 01.1994, p. 84-92.

Research output: Contribution to journalArticle

Verwest, AM, de Leeuw, WJF, Molijn, AC, Andersen, TI, Börresen, AL, Mullaart, E, Uitterlinden, AG & Vijg, J 1994, 'Genome scanning of breast cancers by two-dimensional DNA typing', British Journal of Cancer, vol. 69, no. 1, pp. 84-92.
Verwest AM, de Leeuw WJF, Molijn AC, Andersen TI, Börresen AL, Mullaart E et al. Genome scanning of breast cancers by two-dimensional DNA typing. British Journal of Cancer. 1994 Jan;69(1):84-92.
Verwest, A. M. ; de Leeuw, W. J F ; Molijn, A. C. ; Andersen, T. I. ; Börresen, A. L. ; Mullaart, E. ; Uitterlinden, A. G. ; Vijg, Jan. / Genome scanning of breast cancers by two-dimensional DNA typing. In: British Journal of Cancer. 1994 ; Vol. 69, No. 1. pp. 84-92.
@article{f4555cd19c5f494184748de481cd9c26,
title = "Genome scanning of breast cancers by two-dimensional DNA typing",
abstract = "We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time. Here we demonstrate the potency of this method for total genome scanning of the tumour genome by analysing a small series of breast cancers. Comparison of tumour and normal DNA from ten breast cancer patients, using two-dimensional DNA typing with four core probes, revealed a considerable number of genomic alterations. In contrast, with Southern blot analysis only a few alterations were observed using the same probes. Most of the changes observed (74{\%}) were deletions (absence of spots in the tumour) while 20{\%} corresponded to amplifications (spots of higher intensity in the tumour) and 5{\%} were new spots (gains). About 10{\%} of the genomic changes detected appeared to occur in the tumours of more than one patient.",
author = "Verwest, {A. M.} and {de Leeuw}, {W. J F} and Molijn, {A. C.} and Andersen, {T. I.} and B{\"o}rresen, {A. L.} and E. Mullaart and Uitterlinden, {A. G.} and Jan Vijg",
year = "1994",
month = "1",
language = "English (US)",
volume = "69",
pages = "84--92",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Genome scanning of breast cancers by two-dimensional DNA typing

AU - Verwest, A. M.

AU - de Leeuw, W. J F

AU - Molijn, A. C.

AU - Andersen, T. I.

AU - Börresen, A. L.

AU - Mullaart, E.

AU - Uitterlinden, A. G.

AU - Vijg, Jan

PY - 1994/1

Y1 - 1994/1

N2 - We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time. Here we demonstrate the potency of this method for total genome scanning of the tumour genome by analysing a small series of breast cancers. Comparison of tumour and normal DNA from ten breast cancer patients, using two-dimensional DNA typing with four core probes, revealed a considerable number of genomic alterations. In contrast, with Southern blot analysis only a few alterations were observed using the same probes. Most of the changes observed (74%) were deletions (absence of spots in the tumour) while 20% corresponded to amplifications (spots of higher intensity in the tumour) and 5% were new spots (gains). About 10% of the genomic changes detected appeared to occur in the tumours of more than one patient.

AB - We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time. Here we demonstrate the potency of this method for total genome scanning of the tumour genome by analysing a small series of breast cancers. Comparison of tumour and normal DNA from ten breast cancer patients, using two-dimensional DNA typing with four core probes, revealed a considerable number of genomic alterations. In contrast, with Southern blot analysis only a few alterations were observed using the same probes. Most of the changes observed (74%) were deletions (absence of spots in the tumour) while 20% corresponded to amplifications (spots of higher intensity in the tumour) and 5% were new spots (gains). About 10% of the genomic changes detected appeared to occur in the tumours of more than one patient.

UR - http://www.scopus.com/inward/record.url?scp=0028054779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028054779&partnerID=8YFLogxK

M3 - Article

C2 - 8286216

AN - SCOPUS:0028054779

VL - 69

SP - 84

EP - 92

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 1

ER -