Genome scanning by two‐dimensional DNA typing: The use of repetitive DNA sequences for rapid mapping of genetic traits

André G. Uitterlinden, Eline P. Slagboom, Erick Mullaart, Ingrid Meulenbelt, Jan Vijg

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The existence of repetitive DNA sequences offers the possibility to assess the mammalian genome for individual variation in its entirety rather than at one or only a few sites. In order to fully explore the various sets of mammalian repeat sequences for this purpose, analytical tools are required which allow many if not all individual members of sets of repetitive elements to be resolved and identified in terms of location and allelic variation. We have applied and further developed an electrophoretic system, two‐dimensional DNA typing, which may fulfill these requirements. The two‐dimensional system combines separation of DNA fragments is by size in a neutral gel, with separation by sequence composition in a denaturing gradient gel. By hybridization with minisatellite‐ and simple‐sequence core probes and by inter‐repeat polymerase chain reaction techniques, it is possible to obtain individual ‐ and even chromosomespecific separation patterns that consist of hundreds of spots. Computerized image analysis and matching of such spot patterns allow the rapid assesment of multiple polymorphisms, spread over the genome, to monitor genetic variability in populations. When coupled to databases of polymorphic DNA markers with a known genomic location, two‐dimensional DNA typing can greatly accellerate the mapping of genetic traits in humans, animals, and plants.

Original languageEnglish (US)
Pages (from-to)119-134
Number of pages16
JournalELECTROPHORESIS
Volume12
Issue number2-3
DOIs
Publication statusPublished - 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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