Genome organization and characterization of mycobacteriophage Bxb1

José Mediavilla, Shruti Jain, Jordon Kriakov, Michael E. Ford, Robert L. Duda, William R. Jacobs, Roger W. Hendrix, Graham F. Hatfull

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Mycobacteriophage Bxb1 is a temperate phage of Mycobacterium smegmatis. The morphology of Bxb1 particles is similar to that of mycobacteriophages L5 and D29, although Bxb1 differs from these phages in other respects. First, it is heteroimmune with L5 and efficiently forms plaques on an L5 lysogen. Secondly, it has a different host range and fails to infect slow-growing mycobacteria, using a receptor system that is apparently different from that of L5 and D29. Thirdly, it is the first mycobacteriophage to be described that forms a large prominent halo around plaques on a lawn of M. smegmatis. The sequence of the Bxb1 genome shows that it possesses a similar overall organization to the genomes of L5 and D29 and shares weak but detectable DNA sequence similarity to these phages within the structural genes. However, Bxb1 uses a different system of integration and excision, a repressor with different specificity to that of L5 and encodes a large number of novel gene products including several with enzymatic functions that could degrade or modify the mycobacterial cell wall.

Original languageEnglish (US)
Pages (from-to)955-970
Number of pages16
JournalMolecular Microbiology
Volume38
Issue number5
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Mediavilla, J., Jain, S., Kriakov, J., Ford, M. E., Duda, R. L., Jacobs, W. R., Hendrix, R. W., & Hatfull, G. F. (2000). Genome organization and characterization of mycobacteriophage Bxb1. Molecular Microbiology, 38(5), 955-970. https://doi.org/10.1046/j.1365-2958.2000.02183.x