Genetic Techniques in Trypanosoma cruzi

Martin C. Taylor, Huan Huang, John M. Kelly

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations

Abstract

It is almost 20 years since genetic manipulation of Trypanosoma cruzi was first reported. In this time, there have been steady improvements in the available vector systems, and the applications of the technology have been extended into new areas. Episomal vectors have been modified to enhance the level of expression of transfected genes and to facilitate the sub-cellular location of their products. Integrative vectors have been adapted to allow the development of inducible expression systems and the construction of vectors which enable genome modification through telomere-associated chromosome fragmentation. The uses of reverse genetic approaches to dissect peroxide metabolism and the mechanisms of drug activity and resistance in T. cruzi are illustrated in this chapter as examples of how the technology has been used to investigate biological function. Although there remains scope to improve the flexibility of these systems, they have made valuable contributions towards exploiting the genome sequence data and providing a greater understanding of parasite biology and the mechanisms of infection.

Original languageEnglish (US)
Title of host publicationAdvances in Parasitology
PublisherAcademic Press
Pages231-250
Number of pages20
DOIs
StatePublished - 2011

Publication series

NameAdvances in Parasitology
Volume75
ISSN (Print)0065-308X

Keywords

  • Functional analysis
  • Genetic manipulation
  • Transfection
  • Transgenic
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Parasitology

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  • Cite this

    Taylor, M. C., Huang, H., & Kelly, J. M. (2011). Genetic Techniques in Trypanosoma cruzi. In Advances in Parasitology (pp. 231-250). (Advances in Parasitology; Vol. 75). Academic Press. https://doi.org/10.1016/B978-0-12-385863-4.00011-3