Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1

F. Chen, B. P. Currie, L. C. Chen, S. G. Spitzer, E. D. Spitzer, A. Casadevall

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cryptococcus neoformans isolates from eight patients with cryptococcal infection were previously assigned into three groups on the basis of repetitive DNA probe (CNRE-1) restriction fragment length polymorphisms. These groups accounted for a disproportionate number of recent clinical isolates in New York City. To further examine the genetic relatedness of isolates within and across CNRE-1 groups, the DNA sequence of the 779-base URA5 gene from each strain was amplified and sequenced. The number of nucleotide differences in pairwise comparisons ranged from 0 to 30 (0 to 3% of the total sequence). Most of the nucleotide differences occurred in the third codon position or in introns. Pairwise comparisons revealed average nucleotide differences within a CNRE-1 group of 4.8 ± 2.6 (n = 8) and between CNRE-1 groups of 21.9 ± 7.0 (n = 20) (P < 0.001). Analysis of URA5 sequences defined three groups that were congruent with those defined by CNRE-1 restriction fragment length polymorphisms. PCR amplification of an rDNA intergenic spacer revealed conservation of the intergenic spacer length within groups. Electrophoretic karyotyping did not distinguish between two isolates in each of two CNRE-1 groups. DNA from all isolates studied hybridized to an α mating type-specific probe. We interpret these results as suggesting a clonal population structure for some pathogenic isolates of C. neoformans in New York City.

Original languageEnglish (US)
Pages (from-to)2818-2822
Number of pages5
JournalJournal of Clinical Microbiology
Volume33
Issue number11
StatePublished - 1995

Fingerprint

Cryptococcus neoformans
DNA Probes
Nucleotides
Restriction Fragment Length Polymorphisms
Karyotyping
Ribosomal DNA
Codon
Introns
Sequence Analysis
Polymerase Chain Reaction
DNA
Infection
Population
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Chen, F., Currie, B. P., Chen, L. C., Spitzer, S. G., Spitzer, E. D., & Casadevall, A. (1995). Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1. Journal of Clinical Microbiology, 33(11), 2818-2822.

Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1. / Chen, F.; Currie, B. P.; Chen, L. C.; Spitzer, S. G.; Spitzer, E. D.; Casadevall, A.

In: Journal of Clinical Microbiology, Vol. 33, No. 11, 1995, p. 2818-2822.

Research output: Contribution to journalArticle

Chen, F, Currie, BP, Chen, LC, Spitzer, SG, Spitzer, ED & Casadevall, A 1995, 'Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1', Journal of Clinical Microbiology, vol. 33, no. 11, pp. 2818-2822.
Chen, F. ; Currie, B. P. ; Chen, L. C. ; Spitzer, S. G. ; Spitzer, E. D. ; Casadevall, A. / Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1. In: Journal of Clinical Microbiology. 1995 ; Vol. 33, No. 11. pp. 2818-2822.
@article{10af1ea6ccb54984b89284b73aa850cd,
title = "Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1",
abstract = "Cryptococcus neoformans isolates from eight patients with cryptococcal infection were previously assigned into three groups on the basis of repetitive DNA probe (CNRE-1) restriction fragment length polymorphisms. These groups accounted for a disproportionate number of recent clinical isolates in New York City. To further examine the genetic relatedness of isolates within and across CNRE-1 groups, the DNA sequence of the 779-base URA5 gene from each strain was amplified and sequenced. The number of nucleotide differences in pairwise comparisons ranged from 0 to 30 (0 to 3{\%} of the total sequence). Most of the nucleotide differences occurred in the third codon position or in introns. Pairwise comparisons revealed average nucleotide differences within a CNRE-1 group of 4.8 ± 2.6 (n = 8) and between CNRE-1 groups of 21.9 ± 7.0 (n = 20) (P < 0.001). Analysis of URA5 sequences defined three groups that were congruent with those defined by CNRE-1 restriction fragment length polymorphisms. PCR amplification of an rDNA intergenic spacer revealed conservation of the intergenic spacer length within groups. Electrophoretic karyotyping did not distinguish between two isolates in each of two CNRE-1 groups. DNA from all isolates studied hybridized to an α mating type-specific probe. We interpret these results as suggesting a clonal population structure for some pathogenic isolates of C. neoformans in New York City.",
author = "F. Chen and Currie, {B. P.} and Chen, {L. C.} and Spitzer, {S. G.} and Spitzer, {E. D.} and A. Casadevall",
year = "1995",
language = "English (US)",
volume = "33",
pages = "2818--2822",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Genetic relatedness of Cryptococcus neoformans clinical isolates grouped with the repetitive DNA probe CNRE-1

AU - Chen, F.

AU - Currie, B. P.

AU - Chen, L. C.

AU - Spitzer, S. G.

AU - Spitzer, E. D.

AU - Casadevall, A.

PY - 1995

Y1 - 1995

N2 - Cryptococcus neoformans isolates from eight patients with cryptococcal infection were previously assigned into three groups on the basis of repetitive DNA probe (CNRE-1) restriction fragment length polymorphisms. These groups accounted for a disproportionate number of recent clinical isolates in New York City. To further examine the genetic relatedness of isolates within and across CNRE-1 groups, the DNA sequence of the 779-base URA5 gene from each strain was amplified and sequenced. The number of nucleotide differences in pairwise comparisons ranged from 0 to 30 (0 to 3% of the total sequence). Most of the nucleotide differences occurred in the third codon position or in introns. Pairwise comparisons revealed average nucleotide differences within a CNRE-1 group of 4.8 ± 2.6 (n = 8) and between CNRE-1 groups of 21.9 ± 7.0 (n = 20) (P < 0.001). Analysis of URA5 sequences defined three groups that were congruent with those defined by CNRE-1 restriction fragment length polymorphisms. PCR amplification of an rDNA intergenic spacer revealed conservation of the intergenic spacer length within groups. Electrophoretic karyotyping did not distinguish between two isolates in each of two CNRE-1 groups. DNA from all isolates studied hybridized to an α mating type-specific probe. We interpret these results as suggesting a clonal population structure for some pathogenic isolates of C. neoformans in New York City.

AB - Cryptococcus neoformans isolates from eight patients with cryptococcal infection were previously assigned into three groups on the basis of repetitive DNA probe (CNRE-1) restriction fragment length polymorphisms. These groups accounted for a disproportionate number of recent clinical isolates in New York City. To further examine the genetic relatedness of isolates within and across CNRE-1 groups, the DNA sequence of the 779-base URA5 gene from each strain was amplified and sequenced. The number of nucleotide differences in pairwise comparisons ranged from 0 to 30 (0 to 3% of the total sequence). Most of the nucleotide differences occurred in the third codon position or in introns. Pairwise comparisons revealed average nucleotide differences within a CNRE-1 group of 4.8 ± 2.6 (n = 8) and between CNRE-1 groups of 21.9 ± 7.0 (n = 20) (P < 0.001). Analysis of URA5 sequences defined three groups that were congruent with those defined by CNRE-1 restriction fragment length polymorphisms. PCR amplification of an rDNA intergenic spacer revealed conservation of the intergenic spacer length within groups. Electrophoretic karyotyping did not distinguish between two isolates in each of two CNRE-1 groups. DNA from all isolates studied hybridized to an α mating type-specific probe. We interpret these results as suggesting a clonal population structure for some pathogenic isolates of C. neoformans in New York City.

UR - http://www.scopus.com/inward/record.url?scp=0028858428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028858428&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 2818

EP - 2822

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -