Genetic mechanism accounting for precise immunoglobulin domain deletion in a variant of MPC 11 myeloma cells

Amy L. Kenter, Barbara K. Birshtein

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A variant of the MPC 11 cell line, M 311, produces a short immunoglobulin heavy chain. When compared with the parental γ2b heavy chain, M 311 was found to have a carboxyl terminal deletion comprising the C H3 domain. The COOH-terminal cyanogen bromide (CNBr) cleavage fragment of M 311 is identical to a corresponding segment of a parental heavy chain CNBr fragment, with the exception of a substitution of asparagine for lysine at the COOH-terminal residue. This observation enabled prediction of both the parental DNA sequence in this region and the genetic mechanism which generated the variant, a frameshift followed by premature termination. This hypothesis is supported by studies of the DNA sequence of the MPC 11 γ2b constant region gene.

Original languageEnglish (US)
Pages (from-to)1307-1309
Number of pages3
JournalScience
Volume206
Issue number4424
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • General

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