Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans

Tamar Gattegno, Aditya Mittal, Clari Valansi, Ken C.Q. Nguyen, David H. Hall, Leonid V. Chernomordik, Benjamin Podbilewicz

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Developmental cell fusion is found in germlines, muscles, bones, placentae, and stem cells. In Caenorhabditis elegans 300 somatic cells fuse during development. Although there is extensive information on the early intermediates of viral-induced and intracellular membrane fusion, little is known about late stages in membrane fusion. To dissect the pathway of cell fusion in C. elegans embryos, we use genetic and kinetic analyses using live-confocal and electron microscopy. We simultaneously monitor the rates of multiple cell fusions in developing embryos and find kinetically distinct stages of initiation and completion of membrane fusion in the epidermis. The stages of cell fusion are differentially blocked or retarded in eff-1 and idf-1 mutants. We generate kinetic cell fusion maps for embryos grown at different temperatures. Different sides of the same cell differ in their fusogenicity: the left and right membrane domains are fusion-incompetent, whereas the anterior and posterior membrane domains fuse with autonomous kinetics in embryos. All but one cell pair can initiate the formation of the largest syncytium. The first cell fusion does not trigger a wave of orderly fusions in either direction. Ultrastructural studies show that epidermal syncytiogenesis require eff-1 activities to initiate and expand membrane merger.

Original languageEnglish (US)
Pages (from-to)1153-1166
Number of pages14
JournalMolecular Biology of the Cell
Volume18
Issue number4
DOIs
StatePublished - Apr 2007

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Cell Fusion
Caenorhabditis elegans
Epidermis
Membrane Fusion
Embryonic Structures
Intracellular Membranes
Membranes
Giant Cells
Confocal Microscopy
Placenta
Electron Microscopy
Stem Cells
Bone and Bones
Muscles
Temperature

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans. / Gattegno, Tamar; Mittal, Aditya; Valansi, Clari; Nguyen, Ken C.Q.; Hall, David H.; Chernomordik, Leonid V.; Podbilewicz, Benjamin.

In: Molecular Biology of the Cell, Vol. 18, No. 4, 04.2007, p. 1153-1166.

Research output: Contribution to journalArticle

Gattegno, Tamar ; Mittal, Aditya ; Valansi, Clari ; Nguyen, Ken C.Q. ; Hall, David H. ; Chernomordik, Leonid V. ; Podbilewicz, Benjamin. / Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 4. pp. 1153-1166.
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