Genetic alterations in gastrinomas and nonfunctioning pancreatic neuroendocrine tumors: An analysis of p16/MTS1 tumor suppressor gene inactivation

Peter Muscarella, W. Scott Melvin, William E. Fisher, John Foor, E. Christopher Ellison, James G. Herman, William J. Schirmer, Charles L. Hitchcock, Barry R. DeYoung, Christopher M. Weghorst

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119 Scopus citations


Neoplasms of the endocrine pancreas are extremely rare, and molecular mechanisms influencing their development are poorly understood. Nevertheless, gastrinomas have become a paradigm for the study of hormonally active tumors. In the present study, 12 gastrinoma and non-functioning pancreatic neuroendocrine tumor specimens were evaluated for genetic alterations of the p16/MTS1 tumor suppressor gene. DNA extracted from microdissected portions of paraffin-embedded tumor sections were examined for mutations and homozygous deletions using 'Cold' single-strand conformation polymorphism and semiquantitative PCR-based analyses, respectively. Samples were also analyzed for the presence of 5' CpG island hypermethylation using methylation-specific PCR. The p16/MTS1 gene was found to be homozygously deleted in 41.7% of tumors and methylated in 58.3%, but no mutations were identified by single- strand conformation polymorphism analyses. Overall, 91.7% of the specimens demonstrated inactivating alterations in p16/MTS1. These data suggest that transcriptional silencing of p16/MTS1 is a frequent event in these rare and poorly understood tumors.

Original languageEnglish (US)
Pages (from-to)237-240
Number of pages4
JournalCancer Research
Issue number2
Publication statusPublished - Jan 15 1998
Externally publishedYes


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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