Genes affecting cell competition in drosophila

David M. Tyler, Wei Li, Ning Zhuo, Brett Pellock, Nicholas E. Baker

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Cell competition is a homeostatic mechanism that regulates the size attained by growing tissues. We performed an unbiased genetic screen for mutations that permit the survival of cells being competed due to haplo-insufficiency for RpL36. Mutations that protect RpL36 heterozygous clones include the tumor suppressors expanded, hippo, salvador, mats, and warts, which are members of the Warts pathway, the tumor suppressor fat, and a novel tumor-suppressor mutation. Other hyperplastic or neoplastic mutations did not rescue RpL36 heterozygous clones. Most mutations that rescue cell competition elevated Dppsignaling activity, and the Dsmurf mutation that elevates Dpp signaling was also hyperplastic and rescued. Two nonlethal, nonhyperplastic mutations prevent the apoptosis of Minute heterozygous cells and suggest an apoptosis pathway for cell competition . In addition to rescuing RpL36 heterozygous cells, mutations in Warts pathway genes were supercompetitors that could eliminate wild-type cells nearby. The findings show that differences in Warts pathway activity can lead to competition and implicate the Warts pathway, certain other tumor suppressors, and novel cell death components in cell competition, in addition to the Dpp pathway implicated by previous studies. We suggest that cell competition might occur during tumor development in mammals.

Original languageEnglish (US)
Pages (from-to)643-657
Number of pages15
JournalGenetics
Volume175
Issue number2
DOIs
StatePublished - Feb 2007

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Drosophila
Warts
Mutation
Genes
Neoplasms
Clone Cells
Apoptosis
Genetic Suppression
Cellular Structures
Mammals
Cell Survival
Cell Death
Fats

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Genes affecting cell competition in drosophila. / Tyler, David M.; Li, Wei; Zhuo, Ning; Pellock, Brett; Baker, Nicholas E.

In: Genetics, Vol. 175, No. 2, 02.2007, p. 643-657.

Research output: Contribution to journalArticle

Tyler, DM, Li, W, Zhuo, N, Pellock, B & Baker, NE 2007, 'Genes affecting cell competition in drosophila', Genetics, vol. 175, no. 2, pp. 643-657. https://doi.org/10.1534/genetics.106.061929
Tyler, David M. ; Li, Wei ; Zhuo, Ning ; Pellock, Brett ; Baker, Nicholas E. / Genes affecting cell competition in drosophila. In: Genetics. 2007 ; Vol. 175, No. 2. pp. 643-657.
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