Generation of transgenic mice expressing human hemoglobin E

Qiuying Chen, Eric E. Bouhassira, Arnaud Besse, Sandra M. Suzuka, Mary E. Fabry, Ronald L. Nagel, Rhoda Elison Hirsch

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hemoglobin E (HbE, β26 Glu→Lys) is the most common abnormal Hb variant in the world, and found in greatest frequency in Southeast (SE) Asia. In the United States, HbE is the third most prevalent variant (after HbS and HbC); and its now increasing frequency is due to immigration from SE Asia. HbE homozygotes present a benign clinical picture, but when HbE is coupled with β 0-thalassemia or HbS, variably severe hemoglobinopathies arise. To date, there are no transgenic animal models of HbE-related diseases. We report here the creation of transgenic mice expressing human HbE as a step toward creating animal models for HbE-related diseases. The β E mice exhibit red blood cell hypochromia and target cells consistent with those observed in human patients exhibiting HbE trait. Furthermore, the transgenic HbE hemolysates contain increased amounts of Hb oxidation products.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalBlood Cells, Molecules, and Diseases
Volume33
Issue number3
DOIs
StatePublished - Nov 2004

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Hemoglobin E
Southeastern Asia
Transgenic Mice
Animal Models
Hemoglobinopathies
Genetically Modified Animals
Thalassemia
Emigration and Immigration
Homozygote
Erythrocytes

Keywords

  • Hemoglobin E
  • Homozygote
  • Transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology

Cite this

Chen, Q., Bouhassira, E. E., Besse, A., Suzuka, S. M., Fabry, M. E., Nagel, R. L., & Hirsch, R. E. (2004). Generation of transgenic mice expressing human hemoglobin E. Blood Cells, Molecules, and Diseases, 33(3), 303-307. https://doi.org/10.1016/j.bcmd.2004.07.006

Generation of transgenic mice expressing human hemoglobin E. / Chen, Qiuying; Bouhassira, Eric E.; Besse, Arnaud; Suzuka, Sandra M.; Fabry, Mary E.; Nagel, Ronald L.; Hirsch, Rhoda Elison.

In: Blood Cells, Molecules, and Diseases, Vol. 33, No. 3, 11.2004, p. 303-307.

Research output: Contribution to journalArticle

Chen, Q, Bouhassira, EE, Besse, A, Suzuka, SM, Fabry, ME, Nagel, RL & Hirsch, RE 2004, 'Generation of transgenic mice expressing human hemoglobin E', Blood Cells, Molecules, and Diseases, vol. 33, no. 3, pp. 303-307. https://doi.org/10.1016/j.bcmd.2004.07.006
Chen, Qiuying ; Bouhassira, Eric E. ; Besse, Arnaud ; Suzuka, Sandra M. ; Fabry, Mary E. ; Nagel, Ronald L. ; Hirsch, Rhoda Elison. / Generation of transgenic mice expressing human hemoglobin E. In: Blood Cells, Molecules, and Diseases. 2004 ; Vol. 33, No. 3. pp. 303-307.
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