TY - JOUR
T1 - Generation of IL-3–Secreting CD4+ T Cells by Microbial Challenge at Skin and Mucosal Barriers
AU - Kunnath-Velayudhan, Shajo
AU - Goldberg, Michael F.
AU - Saini, Neeraj K.
AU - Ng, Tony W.
AU - Arora, Pooja
AU - Johndrow, Christopher T.
AU - Saavedra-Avila, Noemi Alejandra
AU - Johnson, Alison J.
AU - Xu, Jiayong
AU - Kim, John
AU - Khajoueinejad, Nazanin
AU - Petro, Christopher D.
AU - Herold, Betsy C.
AU - Lauvau, Gregoire
AU - Chan, John
AU - Jacobs, William R.
AU - Porcelli, Steven A.
N1 - Publisher Copyright:
Copyright © 2019 The Authors
PY - 2019/5/1
Y1 - 2019/5/1
N2 - During Ag priming, naive CD4+ T cells differentiate into subsets with distinct patterns of cytokine expression that dictate to a major extent their functional roles in immune responses. We identified a subset of CD4+ T cells defined by secretion of IL-3 that was induced by Ag stimulation under conditions different from those associated with previously defined functional subsets. Using mouse models of bacterial and viral infections, we showed that IL-3–secreting CD4+ T cells were generated by infection at the skin and mucosa but not by infections introduced directly into the blood. Most IL-3–producing T cells coexpressed GM-CSF and other cytokines that define multifunctionality. Generation of IL-3–secreting T cells in vitro was dependent on IL-1 family cytokines and was inhibited by cytokines that induce canonical Th1 or Th2 cells. Our results identify IL-3–secreting CD4+ T cells as a potential functional subset that arises during priming of naive T cells in specific tissue locations.
AB - During Ag priming, naive CD4+ T cells differentiate into subsets with distinct patterns of cytokine expression that dictate to a major extent their functional roles in immune responses. We identified a subset of CD4+ T cells defined by secretion of IL-3 that was induced by Ag stimulation under conditions different from those associated with previously defined functional subsets. Using mouse models of bacterial and viral infections, we showed that IL-3–secreting CD4+ T cells were generated by infection at the skin and mucosa but not by infections introduced directly into the blood. Most IL-3–producing T cells coexpressed GM-CSF and other cytokines that define multifunctionality. Generation of IL-3–secreting T cells in vitro was dependent on IL-1 family cytokines and was inhibited by cytokines that induce canonical Th1 or Th2 cells. Our results identify IL-3–secreting CD4+ T cells as a potential functional subset that arises during priming of naive T cells in specific tissue locations.
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U2 - 10.4049/immunohorizons.1900028
DO - 10.4049/immunohorizons.1900028
M3 - Article
C2 - 31356170
AN - SCOPUS:85079664394
SN - 2573-7732
VL - 3
SP - 161
EP - 171
JO - ImmunoHorizons
JF - ImmunoHorizons
IS - 5
ER -