Generation of a novel a kinase anchor protein and a myristoylated alanine-rich C kinase substrate-like analog from a single gene

Zhuo Li, Edmund A. Rossi, Jörg D. Hoheisel, Daniel Kalderon, Charles S. Rubin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A unique Drosophila gene encodes two novel signaling proteins. Drosophila A kinase anchor protein 200 (DAKAP200) (753 amino acids) binds regulatory subunits of protein kinase AII (PKAII) isoforms in vitro and in intact cells. The acidic DAKAP200 polypeptide (pI ~3.8) contains an optimal N-terminal myristoylation site and a positively charged domain that resembles the multifunctional phosphorylation site domain of vertebrate myristoylated alanine-rich C kinase substrate proteins. The 15-kilobase pair DAKAP200 gene contains six exons and encodes a second protein, ΔDAKAP200. ΔDAKAP200 is derived from DAKAP200 transcripts by excision of exon 5 (381 codons), which encodes the PKAII binding region and a Pro-rich sequence. ΔAKAP200 appears to be a myristoylated alanine-rich C kinase substrate analog. DAKAP200 and ΔAKAP200 are evident in vivo at all stages of Drosophila development. Thus, both proteins may play important physiological roles throughout the life span of the organism. Nevertheless, DAKAP200 gene expression is regulated. Maximal levels of DAKAP200 are detected in the pupal phase of development; ΔAKAP200 content is elevated 7-fold in adult head (brain) relative to other body parts. Enhancement or suppression of exon 5 excision during DAKAP200 pre-mRNA processing provides potential mechanisms for regulating anchoring of PKAII and targeting of cAMP signals to effector sites in cytoskeleton and/or organelles.

Original languageEnglish (US)
Pages (from-to)27191-27200
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number38
DOIs
StatePublished - Sep 17 1999

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Anchors
Protein Kinases
Phosphotransferases
Genes
Proteins
Exons
Drosophila
Drosophila Akap200 protein
myristoylated alanine-rich C kinase substrate
Phosphorylation
RNA Precursors
Protein Transport
Cytoskeleton
Human Body
Gene expression
Codon
Organelles
Vertebrates
Brain
Protein Isoforms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Generation of a novel a kinase anchor protein and a myristoylated alanine-rich C kinase substrate-like analog from a single gene. / Li, Zhuo; Rossi, Edmund A.; Hoheisel, Jörg D.; Kalderon, Daniel; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 274, No. 38, 17.09.1999, p. 27191-27200.

Research output: Contribution to journalArticle

Li, Zhuo ; Rossi, Edmund A. ; Hoheisel, Jörg D. ; Kalderon, Daniel ; Rubin, Charles S. / Generation of a novel a kinase anchor protein and a myristoylated alanine-rich C kinase substrate-like analog from a single gene. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 38. pp. 27191-27200.
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