Generating S-nitrosothiols from hemoglobin: Mechanisms, conformational dependence, and physiological relevance

Camille J. Roche, Maria B. Cassera, David Dantsker, Rhoda Elison Hirsch, Joel M. Friedman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: The mechanism for production of N2O3 from MetHb, nitrite, and NO is controversial. Results: An Hb intermediate attributed to heme-bound N2O3 is characterized. Conclusion: Partially met-R state Hb can function as a generator of long lived forms of bioactive NO. Significance: The results provide insight into how Hb reactivity with nitrite can be harnessed physiologically and therapeutically.

Original languageEnglish (US)
Pages (from-to)22408-22425
Number of pages18
JournalJournal of Biological Chemistry
Volume288
Issue number31
DOIs
StatePublished - Aug 2 2013

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S-Nitrosothiols
Nitrites
Hemoglobins
Heme

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Generating S-nitrosothiols from hemoglobin : Mechanisms, conformational dependence, and physiological relevance. / Roche, Camille J.; Cassera, Maria B.; Dantsker, David; Hirsch, Rhoda Elison; Friedman, Joel M.

In: Journal of Biological Chemistry, Vol. 288, No. 31, 02.08.2013, p. 22408-22425.

Research output: Contribution to journalArticle

Roche, Camille J. ; Cassera, Maria B. ; Dantsker, David ; Hirsch, Rhoda Elison ; Friedman, Joel M. / Generating S-nitrosothiols from hemoglobin : Mechanisms, conformational dependence, and physiological relevance. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 31. pp. 22408-22425.
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