Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker

Kami Kim, Dominique Soldati, John C. Boothroyd

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

A system for stable transformation of Toxoplasma gondii tachyzoltes was developed that exploited the susceptibility of Toxoplasma to chloramphenicol. Introduction of the chloramphenicol acetyltransferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection resulted in parasites stably expressing CAT. A construct incorporating the tandemly repeated gene, B1, targeted efficiently to its homologous chromosomal locus. Knockout of the single-copy gene, ROP1, was also successful. Stable transformation should permit the identification and analysis of Toxoplasma genes important in the interaction of this opportunistic parasite with its host.

Original languageEnglish (US)
Pages (from-to)911-914
Number of pages4
JournalScience
Volume262
Issue number5135
StatePublished - Nov 5 1993
Externally publishedYes

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Chloramphenicol O-Acetyltransferase
Toxoplasma
Chloramphenicol
Genes
Parasites

ASJC Scopus subject areas

  • General

Cite this

Kim, K., Soldati, D., & Boothroyd, J. C. (1993). Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science, 262(5135), 911-914.

Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. / Kim, Kami; Soldati, Dominique; Boothroyd, John C.

In: Science, Vol. 262, No. 5135, 05.11.1993, p. 911-914.

Research output: Contribution to journalArticle

Kim, K, Soldati, D & Boothroyd, JC 1993, 'Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker', Science, vol. 262, no. 5135, pp. 911-914.
Kim, Kami ; Soldati, Dominique ; Boothroyd, John C. / Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. In: Science. 1993 ; Vol. 262, No. 5135. pp. 911-914.
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