Gene expression variability as a unifying element of the pluripotency network

Elizabeth A. Mason, Jessica C. Mar, Andrew L. Laslett, Martin F. Pera, John Quackenbush, Ernst Wolvetang, Christine A. Wells

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Heterogeneity is a hallmark of stem cell populations, in part due to the molecular differences between cells undergoing self-renewal and those poised to differentiate. We examined phenotypic and molecular heterogeneity in pluripotent stem cell populations, using public gene expression data sets. A high degree of concordance was observed between global gene expression variability and the reported heterogeneity of different human pluripotent lines. Network analysis demonstrated that low-variability genes were the most highly connected, suggesting that these are the most stable elements of the gene regulatory network and are under the highest regulatory constraints. Known drivers of pluripotency were among these, with lowest expression variability of POU5F1 in cells with the highest capacity for self-renewal. Variability of gene expression provides a reliable measure of phenotypic and molecular heterogeneity and predicts those genes with the highest degree of regulatory constraint within the pluripotency network.

Original languageEnglish (US)
Pages (from-to)365-377
Number of pages13
JournalStem Cell Reports
Volume3
Issue number2
DOIs
StatePublished - Aug 12 2014

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Gene expression
Genes
Stem cells
Gene Expression
Pluripotent Stem Cells
Gene Regulatory Networks
Electric network analysis
Population
Stem Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics
  • Medicine(all)

Cite this

Mason, E. A., Mar, J. C., Laslett, A. L., Pera, M. F., Quackenbush, J., Wolvetang, E., & Wells, C. A. (2014). Gene expression variability as a unifying element of the pluripotency network. Stem Cell Reports, 3(2), 365-377. https://doi.org/10.1016/j.stemcr.2014.06.008

Gene expression variability as a unifying element of the pluripotency network. / Mason, Elizabeth A.; Mar, Jessica C.; Laslett, Andrew L.; Pera, Martin F.; Quackenbush, John; Wolvetang, Ernst; Wells, Christine A.

In: Stem Cell Reports, Vol. 3, No. 2, 12.08.2014, p. 365-377.

Research output: Contribution to journalArticle

Mason, EA, Mar, JC, Laslett, AL, Pera, MF, Quackenbush, J, Wolvetang, E & Wells, CA 2014, 'Gene expression variability as a unifying element of the pluripotency network', Stem Cell Reports, vol. 3, no. 2, pp. 365-377. https://doi.org/10.1016/j.stemcr.2014.06.008
Mason EA, Mar JC, Laslett AL, Pera MF, Quackenbush J, Wolvetang E et al. Gene expression variability as a unifying element of the pluripotency network. Stem Cell Reports. 2014 Aug 12;3(2):365-377. https://doi.org/10.1016/j.stemcr.2014.06.008
Mason, Elizabeth A. ; Mar, Jessica C. ; Laslett, Andrew L. ; Pera, Martin F. ; Quackenbush, John ; Wolvetang, Ernst ; Wells, Christine A. / Gene expression variability as a unifying element of the pluripotency network. In: Stem Cell Reports. 2014 ; Vol. 3, No. 2. pp. 365-377.
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