Gene expression profiling in single cells within tissue

Paola Capodieci, Michael Donovan, Heidi Buchinsky, Yusuf Jeffers, Carlos Cordon-Cardo, William Gerald, Jon Edelson, Shailesh M. Shenoy, Robert H. Singer

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

Original languageEnglish (US)
Pages (from-to)663-665
Number of pages3
JournalNature Methods
Volume2
Issue number9
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Capodieci, P., Donovan, M., Buchinsky, H., Jeffers, Y., Cordon-Cardo, C., Gerald, W., Edelson, J., Shenoy, S. M., & Singer, R. H. (2005). Gene expression profiling in single cells within tissue. Nature Methods, 2(9), 663-665. https://doi.org/10.1038/nmeth786