Gene expression profiling in single cells within tissue

Paola Capodieci; Michael Donovan; Heidi Buchinsky; Yusuf Jeffers; Carlos Cordon-Cardo; William Gerald; Jon Edelson; Shailesh M. Shenoy; Robert H. Singer

doi:10.1038/nmeth786

Gene expression profiling in single cells within tissue

Paola Capodieci, Michael Donovan, Heidi Buchinsky, Yusuf Jeffers, Carlos Cordon-Cardo, William Gerald, Jon Edelson, Shailesh M. Shenoy, Robert H. Singer

Cell Biology

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

Original language	English (US)
Pages (from-to)	663-665
Number of pages	3
Journal	Nature Methods
Volume	2
Issue number	9
DOIs	https://doi.org/10.1038/nmeth786
State	Published - Sep 2005

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth786

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title = "Gene expression profiling in single cells within tissue",

abstract = "We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.",

author = "Paola Capodieci and Michael Donovan and Heidi Buchinsky and Yusuf Jeffers and Carlos Cordon-Cardo and William Gerald and Jon Edelson and Shenoy, {Shailesh M.} and Singer, {Robert H.}",

note = "Funding Information: This work was supported in part by US National Institutes of Health CA-R33-083208 and by Aureon Laboratories.",

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AU - Capodieci, Paola

AU - Donovan, Michael

AU - Buchinsky, Heidi

AU - Jeffers, Yusuf

AU - Cordon-Cardo, Carlos

AU - Gerald, William

AU - Edelson, Jon

AU - Shenoy, Shailesh M.

AU - Singer, Robert H.

N1 - Funding Information: This work was supported in part by US National Institutes of Health CA-R33-083208 and by Aureon Laboratories.

PY - 2005/9

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N2 - We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

AB - We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

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Gene expression profiling in single cells within tissue

Abstract

ASJC Scopus subject areas

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