Gene expression profiling in single cells within tissue

Paola Capodieci, Michael Donovan, Heidi Buchinsky, Yusuf Jeffers, Carlos Cordon-Cardo, William Gerald, Jon Edelson, Shailesh M. Shenoy, Robert H. Singer

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

Original languageEnglish (US)
Pages (from-to)663-665
Number of pages3
JournalNature Methods
Volume2
Issue number9
DOIs
StatePublished - Sep 2005

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Gene Expression Profiling
Fluorescence In Situ Hybridization
Transcriptome
Paraffin
Formaldehyde
RNA
Gene Expression

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Capodieci, P., Donovan, M., Buchinsky, H., Jeffers, Y., Cordon-Cardo, C., Gerald, W., ... Singer, R. H. (2005). Gene expression profiling in single cells within tissue. Nature Methods, 2(9), 663-665. https://doi.org/10.1038/nmeth786

Gene expression profiling in single cells within tissue. / Capodieci, Paola; Donovan, Michael; Buchinsky, Heidi; Jeffers, Yusuf; Cordon-Cardo, Carlos; Gerald, William; Edelson, Jon; Shenoy, Shailesh M.; Singer, Robert H.

In: Nature Methods, Vol. 2, No. 9, 09.2005, p. 663-665.

Research output: Contribution to journalArticle

Capodieci, P, Donovan, M, Buchinsky, H, Jeffers, Y, Cordon-Cardo, C, Gerald, W, Edelson, J, Shenoy, SM & Singer, RH 2005, 'Gene expression profiling in single cells within tissue', Nature Methods, vol. 2, no. 9, pp. 663-665. https://doi.org/10.1038/nmeth786
Capodieci P, Donovan M, Buchinsky H, Jeffers Y, Cordon-Cardo C, Gerald W et al. Gene expression profiling in single cells within tissue. Nature Methods. 2005 Sep;2(9):663-665. https://doi.org/10.1038/nmeth786
Capodieci, Paola ; Donovan, Michael ; Buchinsky, Heidi ; Jeffers, Yusuf ; Cordon-Cardo, Carlos ; Gerald, William ; Edelson, Jon ; Shenoy, Shailesh M. ; Singer, Robert H. / Gene expression profiling in single cells within tissue. In: Nature Methods. 2005 ; Vol. 2, No. 9. pp. 663-665.
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