Gene delivery to embryonic stem cells

Naoya Kobayashi, Jorge David Rivas-Carrillo, Alejandro Soto-Gutierrez, Takuya Fukazawa, Yong Chen, Nalu Navarro-Alvarez, Noriaki Tanaka

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Since the establishment of embryonic stem (ES) cells and the identification of tissue-specific stem cells, researchers have made great strides in the analysis of the natural biology of such stem cells for the development of therapeutic applications. Specifically, ES cells are capable of differentiating into all of the cell types that constitute the whole body. Thus, ES cell research promises new type of treatments and possible cures for a variety of debilitating diseases and injuries. The potential medical benefits obtained from stem cell technology are compelling and stem cell research sees a bright future. Control of the growth and differentiation of stem cells is a critical tool in the fields of regenerative medicine, tissue engineering, drug discovery, and toxicity testing. Toward such a goal, we present here an overview of gene delivery in ES cells, covering the following topics: significance of gene delivery in ES cells, stable versus transient gene delivery, cytotoxicity, suspension versus adherent cells, expertise, time, cost, viral vectors for gene transduction (lentiviruses, adenoviruses, and adeno-associated viruses, chemical methods for gene delivery, and mechanical or physical gene delivery methods (electroporation. nucleofection, microinjection, and nuclear transfer).

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalBirth Defects Research Part C - Embryo Today: Reviews
Volume75
Issue number1
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Stem Cells
Stem Cell Research
Genes
Dependovirus
Lentivirus
Regenerative Medicine
Electroporation
Viral Genes
Microinjections
Drug Discovery
Tissue Engineering
Drug-Related Side Effects and Adverse Reactions
Adenoviridae
Suspensions
Research Personnel
Technology
Costs and Cost Analysis
Wounds and Injuries
Therapeutics

Keywords

  • Embryonic stem cells
  • Gene delivery

ASJC Scopus subject areas

  • Embryology

Cite this

Kobayashi, N., Rivas-Carrillo, J. D., Soto-Gutierrez, A., Fukazawa, T., Chen, Y., Navarro-Alvarez, N., & Tanaka, N. (2005). Gene delivery to embryonic stem cells. Birth Defects Research Part C - Embryo Today: Reviews, 75(1), 10-18. https://doi.org/10.1002/bdrc.20031

Gene delivery to embryonic stem cells. / Kobayashi, Naoya; Rivas-Carrillo, Jorge David; Soto-Gutierrez, Alejandro; Fukazawa, Takuya; Chen, Yong; Navarro-Alvarez, Nalu; Tanaka, Noriaki.

In: Birth Defects Research Part C - Embryo Today: Reviews, Vol. 75, No. 1, 03.2005, p. 10-18.

Research output: Contribution to journalArticle

Kobayashi, N, Rivas-Carrillo, JD, Soto-Gutierrez, A, Fukazawa, T, Chen, Y, Navarro-Alvarez, N & Tanaka, N 2005, 'Gene delivery to embryonic stem cells', Birth Defects Research Part C - Embryo Today: Reviews, vol. 75, no. 1, pp. 10-18. https://doi.org/10.1002/bdrc.20031
Kobayashi N, Rivas-Carrillo JD, Soto-Gutierrez A, Fukazawa T, Chen Y, Navarro-Alvarez N et al. Gene delivery to embryonic stem cells. Birth Defects Research Part C - Embryo Today: Reviews. 2005 Mar;75(1):10-18. https://doi.org/10.1002/bdrc.20031
Kobayashi, Naoya ; Rivas-Carrillo, Jorge David ; Soto-Gutierrez, Alejandro ; Fukazawa, Takuya ; Chen, Yong ; Navarro-Alvarez, Nalu ; Tanaka, Noriaki. / Gene delivery to embryonic stem cells. In: Birth Defects Research Part C - Embryo Today: Reviews. 2005 ; Vol. 75, No. 1. pp. 10-18.
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