GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila

Aaron J. Bell, Charles Guerra, Vincent Phung, Saraswathy Nair, Raviraja Seetharam, Peter Satir

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ciliary guanine nucleotide exchange factors (GEFs) potentially activate G proteins in intraflagellar transport (IFT) cargo release. Several classes of GEFs have been localized to cilia or basal bodies and shown to be functionally important in the prevention of ciliopathies, but ciliary Arl-type Sec 7 related GEFs have not been well characterized. Nair et al. [1999] identified a Paramecium ciliary Sec7 GEF, PSec7. In Tetrahymena, Gef1p (GEF1), tentatively identified by PSec7 antibody, possesses ciliary and nuclear targeting sequences and like PSec7 localizes to cilia and macronuclei. Upregulation of GEF1 RNA followed deciliation and subsequent ciliary regrowth. Corresponding to similar Psec7 domains, GEF1domains contain IQ-like motifs and putative PH domains, in addition to GBF/BIG canonical motifs. Genomic analysis identified two additional Tetrahymena GBF/BIG Sec7 family GEFs (GEF2, GEF3), which do not possess ciliary targeting sequences. GEF1 and GEF2 were HA modified to determine cellular localization. Cells transformed to produce appropriately truncated GEF1-HA showed localization to somatic and oral cilia, but not to macronuclei. Subtle defects in ciliary stability and function were detected. GEF2-HA localized near basal bodies but not to cilia. These results indicate that GEF1 is the resident Tetrahymena ciliary protein orthologous to PSec7.

Original languageEnglish (US)
Pages (from-to)483-499
Number of pages17
JournalCell Motility and the Cytoskeleton
Volume66
Issue number8
DOIs
StatePublished - Aug 2009

Fingerprint

Tetrahymena thermophila
Cilia
Guanine Nucleotide Exchange Factors
Tetrahymena
Macronucleus
Basal Bodies
Paramecium
GTP-Binding Proteins
Up-Regulation
RNA
Sec7 guanine nucleotide exchange factors
Antibodies
Proteins

Keywords

  • Arl G proteins
  • Cilia
  • GEF
  • IFT
  • IQ motifs
  • PH domains

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Bell, A. J., Guerra, C., Phung, V., Nair, S., Seetharam, R., & Satir, P. (2009). GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila. Cell Motility and the Cytoskeleton, 66(8), 483-499. https://doi.org/10.1002/cm.20348

GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila. / Bell, Aaron J.; Guerra, Charles; Phung, Vincent; Nair, Saraswathy; Seetharam, Raviraja; Satir, Peter.

In: Cell Motility and the Cytoskeleton, Vol. 66, No. 8, 08.2009, p. 483-499.

Research output: Contribution to journalArticle

Bell, AJ, Guerra, C, Phung, V, Nair, S, Seetharam, R & Satir, P 2009, 'GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila', Cell Motility and the Cytoskeleton, vol. 66, no. 8, pp. 483-499. https://doi.org/10.1002/cm.20348
Bell AJ, Guerra C, Phung V, Nair S, Seetharam R, Satir P. GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila. Cell Motility and the Cytoskeleton. 2009 Aug;66(8):483-499. https://doi.org/10.1002/cm.20348
Bell, Aaron J. ; Guerra, Charles ; Phung, Vincent ; Nair, Saraswathy ; Seetharam, Raviraja ; Satir, Peter. / GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila. In: Cell Motility and the Cytoskeleton. 2009 ; Vol. 66, No. 8. pp. 483-499.
@article{f0c1a53a965b4784a9e419f3a61afe99,
title = "GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila",
abstract = "Ciliary guanine nucleotide exchange factors (GEFs) potentially activate G proteins in intraflagellar transport (IFT) cargo release. Several classes of GEFs have been localized to cilia or basal bodies and shown to be functionally important in the prevention of ciliopathies, but ciliary Arl-type Sec 7 related GEFs have not been well characterized. Nair et al. [1999] identified a Paramecium ciliary Sec7 GEF, PSec7. In Tetrahymena, Gef1p (GEF1), tentatively identified by PSec7 antibody, possesses ciliary and nuclear targeting sequences and like PSec7 localizes to cilia and macronuclei. Upregulation of GEF1 RNA followed deciliation and subsequent ciliary regrowth. Corresponding to similar Psec7 domains, GEF1domains contain IQ-like motifs and putative PH domains, in addition to GBF/BIG canonical motifs. Genomic analysis identified two additional Tetrahymena GBF/BIG Sec7 family GEFs (GEF2, GEF3), which do not possess ciliary targeting sequences. GEF1 and GEF2 were HA modified to determine cellular localization. Cells transformed to produce appropriately truncated GEF1-HA showed localization to somatic and oral cilia, but not to macronuclei. Subtle defects in ciliary stability and function were detected. GEF2-HA localized near basal bodies but not to cilia. These results indicate that GEF1 is the resident Tetrahymena ciliary protein orthologous to PSec7.",
keywords = "Arl G proteins, Cilia, GEF, IFT, IQ motifs, PH domains",
author = "Bell, {Aaron J.} and Charles Guerra and Vincent Phung and Saraswathy Nair and Raviraja Seetharam and Peter Satir",
year = "2009",
month = "8",
doi = "10.1002/cm.20348",
language = "English (US)",
volume = "66",
pages = "483--499",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - GEF1 is a ciliary Sec7 GEF of Tetrahymena thermophila

AU - Bell, Aaron J.

AU - Guerra, Charles

AU - Phung, Vincent

AU - Nair, Saraswathy

AU - Seetharam, Raviraja

AU - Satir, Peter

PY - 2009/8

Y1 - 2009/8

N2 - Ciliary guanine nucleotide exchange factors (GEFs) potentially activate G proteins in intraflagellar transport (IFT) cargo release. Several classes of GEFs have been localized to cilia or basal bodies and shown to be functionally important in the prevention of ciliopathies, but ciliary Arl-type Sec 7 related GEFs have not been well characterized. Nair et al. [1999] identified a Paramecium ciliary Sec7 GEF, PSec7. In Tetrahymena, Gef1p (GEF1), tentatively identified by PSec7 antibody, possesses ciliary and nuclear targeting sequences and like PSec7 localizes to cilia and macronuclei. Upregulation of GEF1 RNA followed deciliation and subsequent ciliary regrowth. Corresponding to similar Psec7 domains, GEF1domains contain IQ-like motifs and putative PH domains, in addition to GBF/BIG canonical motifs. Genomic analysis identified two additional Tetrahymena GBF/BIG Sec7 family GEFs (GEF2, GEF3), which do not possess ciliary targeting sequences. GEF1 and GEF2 were HA modified to determine cellular localization. Cells transformed to produce appropriately truncated GEF1-HA showed localization to somatic and oral cilia, but not to macronuclei. Subtle defects in ciliary stability and function were detected. GEF2-HA localized near basal bodies but not to cilia. These results indicate that GEF1 is the resident Tetrahymena ciliary protein orthologous to PSec7.

AB - Ciliary guanine nucleotide exchange factors (GEFs) potentially activate G proteins in intraflagellar transport (IFT) cargo release. Several classes of GEFs have been localized to cilia or basal bodies and shown to be functionally important in the prevention of ciliopathies, but ciliary Arl-type Sec 7 related GEFs have not been well characterized. Nair et al. [1999] identified a Paramecium ciliary Sec7 GEF, PSec7. In Tetrahymena, Gef1p (GEF1), tentatively identified by PSec7 antibody, possesses ciliary and nuclear targeting sequences and like PSec7 localizes to cilia and macronuclei. Upregulation of GEF1 RNA followed deciliation and subsequent ciliary regrowth. Corresponding to similar Psec7 domains, GEF1domains contain IQ-like motifs and putative PH domains, in addition to GBF/BIG canonical motifs. Genomic analysis identified two additional Tetrahymena GBF/BIG Sec7 family GEFs (GEF2, GEF3), which do not possess ciliary targeting sequences. GEF1 and GEF2 were HA modified to determine cellular localization. Cells transformed to produce appropriately truncated GEF1-HA showed localization to somatic and oral cilia, but not to macronuclei. Subtle defects in ciliary stability and function were detected. GEF2-HA localized near basal bodies but not to cilia. These results indicate that GEF1 is the resident Tetrahymena ciliary protein orthologous to PSec7.

KW - Arl G proteins

KW - Cilia

KW - GEF

KW - IFT

KW - IQ motifs

KW - PH domains

UR - http://www.scopus.com/inward/record.url?scp=67849116445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67849116445&partnerID=8YFLogxK

U2 - 10.1002/cm.20348

DO - 10.1002/cm.20348

M3 - Article

C2 - 19267341

AN - SCOPUS:67849116445

VL - 66

SP - 483

EP - 499

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 8

ER -