Gating of gap junction channels.

David C. Spray, R. L. White, A. C. de Carvalho, A. L. Harris, Michael V. L. Bennett

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Gap junctional conductance ( gj ) in various species is gated by voltage and intracellular pH (pHi). In amphibian embryos, gj is reduced to half by a 14 mV transjunctional voltage ( Vj ), a change that in fish embryo requires approximately 28 mV. Crayfish septate axon and pairs of dissociated rat myocytes show no voltage dependence of gj over a range of Vj greater than +/- 50 mV. In fish and amphibian blastomeres , gj is steeply decreased by decrease in pHi (n, Hill coefficient: 4.5) and the apparent pKH (7.3) is in the physiological range. In crayfish septate axon the pKH is lower (6.7) and the curve is less steep (n = 2.7). Rises in cytoplasmic Ca can also decrease gj but much higher concentrations are required (greater than 0.1 mM in fish blastomeres). Voltage and pH gates on gap junctions in amphibian embryos appear independent. In squid blastomeres pH gates exhibit some sensitivity to potential, both transjunctional and between inside and outside. A pharmacology of gap junctions is being developed: certain agents block gj directly (aldehydes, alcohols, NEM in crayfish); others block by decreasing pHi (esters that are hydrolyzed by intrinsic esterases, NEM in vertebrates, and, as in the experiments demonstrating the effect of pHi, weak acids). Certain agents block pH sensitivity without affecting voltage dependence (retinoic acid, glutaraldehyde, EEDQ), further indicating separateness of pH and voltage gates. These studies demonstrate a dynamics of gap junctional conductance and variability in gating in a series of possibly homologous membrane channels.

Original languageEnglish (US)
Pages (from-to)219-230
Number of pages12
JournalBiophysical Journal
Volume45
Issue number1
StatePublished - Jan 1984
Externally publishedYes

Fingerprint

Gap Junctions
Blastomeres
Astacoidea
Amphibians
Fishes
Embryonic Structures
Axons
Decapodiformes
Glutaral
Esterases
Tretinoin
Ion Channels
Aldehydes
Muscle Cells
Vertebrates
Esters
Alcohols
Pharmacology
Acids

ASJC Scopus subject areas

  • Biophysics

Cite this

Spray, D. C., White, R. L., de Carvalho, A. C., Harris, A. L., & Bennett, M. V. L. (1984). Gating of gap junction channels. Biophysical Journal, 45(1), 219-230.

Gating of gap junction channels. / Spray, David C.; White, R. L.; de Carvalho, A. C.; Harris, A. L.; Bennett, Michael V. L.

In: Biophysical Journal, Vol. 45, No. 1, 01.1984, p. 219-230.

Research output: Contribution to journalArticle

Spray, DC, White, RL, de Carvalho, AC, Harris, AL & Bennett, MVL 1984, 'Gating of gap junction channels.', Biophysical Journal, vol. 45, no. 1, pp. 219-230.
Spray DC, White RL, de Carvalho AC, Harris AL, Bennett MVL. Gating of gap junction channels. Biophysical Journal. 1984 Jan;45(1):219-230.
Spray, David C. ; White, R. L. ; de Carvalho, A. C. ; Harris, A. L. ; Bennett, Michael V. L. / Gating of gap junction channels. In: Biophysical Journal. 1984 ; Vol. 45, No. 1. pp. 219-230.
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