Gap junctions remain open during cytochrome c-induced cell death

Relationship of conductance to 'bystander' cell killing

K. Cusato, H. Ripps, J. Zakevicius, David C. Spray

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Previous reports have shown that gap junctions relay cell death in many cell types. However, changes in electrical coupling and their dynamics during cell death are poorly understood. We performed comprehensive studies of electrical coupling following induction of cell death by single-cell cytochrome c (cyC) injection in paired Xenopus oocytes. Cell death was rapidly induced by cyC in injected cells, and cell death was also observed in uninjected bystander cells electrically coupled to the cyC-injected oocytes. Gap junction currents either remained at pre-cyC injection levels or increased dramatically as the injected cell died. Nonjunctional currents increased in injected cells immediately following cyC injection; nonjunctional currents increased slowly in uninjected bystander cells. Bystander cell death occurred only when junctional conductance was ∼6 μS. Both 1,2-bis-(o-aminophenoxy)-ethane-N,N,-N′,N′-tetraacetic acid tetraacetoxy-methyl ester and Xestospongin C inhibited bystander cell death in pairs that had reached the death conductance threshold, suggesting that Ca2+ and inositol 1,4,5 triphosphate are involved in the process.

Original languageEnglish (US)
Pages (from-to)1707-1714
Number of pages8
JournalCell Death and Differentiation
Volume13
Issue number10
DOIs
StatePublished - Oct 2006

Fingerprint

Gap Junctions
Cytochromes c
Cell Death
Injections
Oocytes
Ethane
Inositol 1,4,5-Trisphosphate
Xenopus
Acids

Keywords

  • Bystander effect
  • Cell death
  • Gap junctions
  • Intercellular communication
  • Xenopus oocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gap junctions remain open during cytochrome c-induced cell death : Relationship of conductance to 'bystander' cell killing. / Cusato, K.; Ripps, H.; Zakevicius, J.; Spray, David C.

In: Cell Death and Differentiation, Vol. 13, No. 10, 10.2006, p. 1707-1714.

Research output: Contribution to journalArticle

@article{1832241d3d0f47049dd3d8d72f6ce95d,
title = "Gap junctions remain open during cytochrome c-induced cell death: Relationship of conductance to 'bystander' cell killing",
abstract = "Previous reports have shown that gap junctions relay cell death in many cell types. However, changes in electrical coupling and their dynamics during cell death are poorly understood. We performed comprehensive studies of electrical coupling following induction of cell death by single-cell cytochrome c (cyC) injection in paired Xenopus oocytes. Cell death was rapidly induced by cyC in injected cells, and cell death was also observed in uninjected bystander cells electrically coupled to the cyC-injected oocytes. Gap junction currents either remained at pre-cyC injection levels or increased dramatically as the injected cell died. Nonjunctional currents increased in injected cells immediately following cyC injection; nonjunctional currents increased slowly in uninjected bystander cells. Bystander cell death occurred only when junctional conductance was ∼6 μS. Both 1,2-bis-(o-aminophenoxy)-ethane-N,N,-N′,N′-tetraacetic acid tetraacetoxy-methyl ester and Xestospongin C inhibited bystander cell death in pairs that had reached the death conductance threshold, suggesting that Ca2+ and inositol 1,4,5 triphosphate are involved in the process.",
keywords = "Bystander effect, Cell death, Gap junctions, Intercellular communication, Xenopus oocytes",
author = "K. Cusato and H. Ripps and J. Zakevicius and Spray, {David C.}",
year = "2006",
month = "10",
doi = "10.1038/sj.cdd.4401876",
language = "English (US)",
volume = "13",
pages = "1707--1714",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Gap junctions remain open during cytochrome c-induced cell death

T2 - Relationship of conductance to 'bystander' cell killing

AU - Cusato, K.

AU - Ripps, H.

AU - Zakevicius, J.

AU - Spray, David C.

PY - 2006/10

Y1 - 2006/10

N2 - Previous reports have shown that gap junctions relay cell death in many cell types. However, changes in electrical coupling and their dynamics during cell death are poorly understood. We performed comprehensive studies of electrical coupling following induction of cell death by single-cell cytochrome c (cyC) injection in paired Xenopus oocytes. Cell death was rapidly induced by cyC in injected cells, and cell death was also observed in uninjected bystander cells electrically coupled to the cyC-injected oocytes. Gap junction currents either remained at pre-cyC injection levels or increased dramatically as the injected cell died. Nonjunctional currents increased in injected cells immediately following cyC injection; nonjunctional currents increased slowly in uninjected bystander cells. Bystander cell death occurred only when junctional conductance was ∼6 μS. Both 1,2-bis-(o-aminophenoxy)-ethane-N,N,-N′,N′-tetraacetic acid tetraacetoxy-methyl ester and Xestospongin C inhibited bystander cell death in pairs that had reached the death conductance threshold, suggesting that Ca2+ and inositol 1,4,5 triphosphate are involved in the process.

AB - Previous reports have shown that gap junctions relay cell death in many cell types. However, changes in electrical coupling and their dynamics during cell death are poorly understood. We performed comprehensive studies of electrical coupling following induction of cell death by single-cell cytochrome c (cyC) injection in paired Xenopus oocytes. Cell death was rapidly induced by cyC in injected cells, and cell death was also observed in uninjected bystander cells electrically coupled to the cyC-injected oocytes. Gap junction currents either remained at pre-cyC injection levels or increased dramatically as the injected cell died. Nonjunctional currents increased in injected cells immediately following cyC injection; nonjunctional currents increased slowly in uninjected bystander cells. Bystander cell death occurred only when junctional conductance was ∼6 μS. Both 1,2-bis-(o-aminophenoxy)-ethane-N,N,-N′,N′-tetraacetic acid tetraacetoxy-methyl ester and Xestospongin C inhibited bystander cell death in pairs that had reached the death conductance threshold, suggesting that Ca2+ and inositol 1,4,5 triphosphate are involved in the process.

KW - Bystander effect

KW - Cell death

KW - Gap junctions

KW - Intercellular communication

KW - Xenopus oocytes

UR - http://www.scopus.com/inward/record.url?scp=33748681711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748681711&partnerID=8YFLogxK

U2 - 10.1038/sj.cdd.4401876

DO - 10.1038/sj.cdd.4401876

M3 - Article

VL - 13

SP - 1707

EP - 1714

JO - Cell Death and Differentiation

JF - Cell Death and Differentiation

SN - 1350-9047

IS - 10

ER -