Gap junctions in the chicken pineal gland

Viviana M. Berthoud, David H. Hall, Erwin Strahsburger, Eric C. Beyer, Juan C. Sáez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The chicken pineal gland, which contains a heterogeneous cell population, sustains a circadian rhythm of activity. Synchronization of cellular activity of heterogeneous cells might be facilitated by gap junctional intercellular channels which are permeable to ions and second messengers. To test this possibility, we looked for morphologically identifiable gap junctions between the different pineal cells, used antibodies and cDNA probes to screen for the presence of connexins, and tested for functional intercellular coupling. By transmission electron microscopy and immunocytochemistry, gap junctions and connexins were observed between pinealocyte cell bodies, stromal cells, astrocytes, and astrocyte and pinealocyte processes. Two gap junctional proteins, connexin43 and connexin45, were detected by immunocytochemistry, immunoblotting and RNA blot analysis. Functional intercellular coupling was observed in the gland by transfer of low molecular weight dyes. Dye transferred between homologous and heterologous cells. These data suggest that homologous and heterologous gap junctions may provide a mechanism for coordination of the cellular responses of the elements of the biological clock which are induced by lighting cues to produce the circadian rhythm of pineal activity. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)257-270
Number of pages14
JournalBrain Research
Volume861
Issue number2
DOIs
StatePublished - Apr 14 2000

Fingerprint

Pineal Gland
Gap Junctions
Chickens
Connexins
Circadian Rhythm
Astrocytes
Coloring Agents
Immunohistochemistry
Biological Clocks
Connexin 43
Response Elements
Second Messenger Systems
Stromal Cells
Lighting
Transmission Electron Microscopy
Immunoblotting
Cues
Complementary DNA
Molecular Weight
RNA

Keywords

  • Astrocyte
  • Circadian rhythm
  • Connexin
  • Dye coupling
  • Intercellular communication
  • Pinealocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Berthoud, V. M., Hall, D. H., Strahsburger, E., Beyer, E. C., & Sáez, J. C. (2000). Gap junctions in the chicken pineal gland. Brain Research, 861(2), 257-270. https://doi.org/10.1016/S0006-8993(00)01987-9

Gap junctions in the chicken pineal gland. / Berthoud, Viviana M.; Hall, David H.; Strahsburger, Erwin; Beyer, Eric C.; Sáez, Juan C.

In: Brain Research, Vol. 861, No. 2, 14.04.2000, p. 257-270.

Research output: Contribution to journalArticle

Berthoud, VM, Hall, DH, Strahsburger, E, Beyer, EC & Sáez, JC 2000, 'Gap junctions in the chicken pineal gland', Brain Research, vol. 861, no. 2, pp. 257-270. https://doi.org/10.1016/S0006-8993(00)01987-9
Berthoud VM, Hall DH, Strahsburger E, Beyer EC, Sáez JC. Gap junctions in the chicken pineal gland. Brain Research. 2000 Apr 14;861(2):257-270. https://doi.org/10.1016/S0006-8993(00)01987-9
Berthoud, Viviana M. ; Hall, David H. ; Strahsburger, Erwin ; Beyer, Eric C. ; Sáez, Juan C. / Gap junctions in the chicken pineal gland. In: Brain Research. 2000 ; Vol. 861, No. 2. pp. 257-270.
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