Gap junctional channels regulate acid secretion in the mammalian gastric gland

K. Radebold, E. Horakova, J. Gloeckner, G. Ortega, David C. Spray, H. Vieweger, K. Siebert, L. Manuelidis, J. P. Geibel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Gap junction channels are regarded as a primary pathway for intercellular message transfer, including calcium wave propagation. Our study identified two gap junctional proteins, connexin26 and connexin32, in rat gastric glands by RT-PCR, Western blot analysis, and immunofluorescence. We demonstrated a potential physiological role of the gap junctional channels in the acid secretory process using the calcium indicator fluo-3, and microinjection of Lucifer Yellow. Application of gastrin (10-7 M) to the basolateral membrane resulted in the induction of uniphasic calcium signals in adjacent parietal cells. In addition, single parietal cell microinjections in intact glands with the cell-impermeant dye Lucifer Yellow resulted in a transfer of dye from the injected cell to the adjacent parietal cell following gastrin stimulation, demonstrating gastrin-induced cell-to-cell communication. Both calcium wave propagation and Lucifer Yellow transfer were blocked by the gap junction inhibitor 18α-glycyrrhetinic acid. Our studies demonstrate that functional gap junction channels in gastric glands provide an effective means for rapid cell-to-cell communication and allow for the rapid onset of acid secretion.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalJournal of Membrane Biology
Volume183
Issue number3
DOIs
StatePublished - Oct 1 2001

Fingerprint

Gastric Mucosa
Gap Junctions
Acids
Gastrin-Secreting Cells
Calcium Signaling
Microinjections
Cell Communication
Coloring Agents
Glycyrrhetinic Acid
Calcium
Secretory Pathway
Gastrins
Fluorescent Antibody Technique
Western Blotting
Polymerase Chain Reaction
Membranes
lucifer yellow
Proteins

Keywords

  • Calcium signaling
  • Cell-to-cell communication
  • Intracellular calcium
  • Lucifer Yellow
  • Parietal cells
  • Stomach

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Radebold, K., Horakova, E., Gloeckner, J., Ortega, G., Spray, D. C., Vieweger, H., ... Geibel, J. P. (2001). Gap junctional channels regulate acid secretion in the mammalian gastric gland. Journal of Membrane Biology, 183(3), 147-153. https://doi.org/10.1007/s00232-001-0062-9

Gap junctional channels regulate acid secretion in the mammalian gastric gland. / Radebold, K.; Horakova, E.; Gloeckner, J.; Ortega, G.; Spray, David C.; Vieweger, H.; Siebert, K.; Manuelidis, L.; Geibel, J. P.

In: Journal of Membrane Biology, Vol. 183, No. 3, 01.10.2001, p. 147-153.

Research output: Contribution to journalArticle

Radebold, K, Horakova, E, Gloeckner, J, Ortega, G, Spray, DC, Vieweger, H, Siebert, K, Manuelidis, L & Geibel, JP 2001, 'Gap junctional channels regulate acid secretion in the mammalian gastric gland', Journal of Membrane Biology, vol. 183, no. 3, pp. 147-153. https://doi.org/10.1007/s00232-001-0062-9
Radebold, K. ; Horakova, E. ; Gloeckner, J. ; Ortega, G. ; Spray, David C. ; Vieweger, H. ; Siebert, K. ; Manuelidis, L. ; Geibel, J. P. / Gap junctional channels regulate acid secretion in the mammalian gastric gland. In: Journal of Membrane Biology. 2001 ; Vol. 183, No. 3. pp. 147-153.
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