Gap junction disappearance in astrocytes and leptomeningeal cells as a consequence of protozoan infection

Antonio C. De Campos Carvalho, Christine Roy, Elliot L. Hertzberg, Herbert B. Tanowitz, John A. Kessler, Louis M. Weiss, Murray Wittner, Rolf Dermietzel, Yang Gao, David C. Spray

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28 Scopus citations

Abstract

Trypanosoma cruzi and Toxoplasma gondii are protozoan parasites capable of causing infections of the nervous system. In order to determine effects of infection by these organisms on intercellular communication in the brain, dye coupling and connexin abundance and distribution were examined in leptomeningeal cells and astrocytes infected with T. cruzi or T. gondii. For both cell types infected with either type of protozoan parasite, intercellular diffusion of intracellularly injected Lucifer Yellow was dramatically reduced. Immunocytochemistry with antibodies specific for connexin43 (in astrocytes) or both connexin43 and connexin26 (for leptomeningeal cells) demonstrated that punctate gap junctional staining was much reduced in infected cells, although uninfected neighbors could display normal connexin abundance and distribution. Western blot analyses revealed that connexin43 abundance in both cell types infected with either parasite was similar to that in uninfected cells. Phosphorylation state of connexin43 (inferred from electrophoretic mobility of connexin43 isoforms) was not significantly affected by the infection process. Immunocytochemistry of whole brains from animals acutely infected with either parasite also showed a marked reduction in connexin43 expression. We conclude that infection of both types of brain cells with either protozoan parasite results in a loss of intercellular communication and organized gap junction plaques without affecting expression levels or posttranslational processing of gap junction proteins. Presumably, these changes in gap junction distribution result from altered targeting of the junctional protein to the plasma membrane, and/or from changes in assembly of subunits into functional channels.

Original languageEnglish (US)
Pages (from-to)304-314
Number of pages11
JournalBrain Research
Volume790
Issue number1-2
DOIs
StatePublished - Apr 20 1998

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Keywords

  • Connexin
  • Cx26
  • Cx43
  • Dye coupling
  • Glia
  • Toxoplasma gondii
  • Toxoplasmosis
  • Trypomosama cruzi

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

De Campos Carvalho, A. C., Roy, C., Hertzberg, E. L., Tanowitz, H. B., Kessler, J. A., Weiss, L. M., Wittner, M., Dermietzel, R., Gao, Y., & Spray, D. C. (1998). Gap junction disappearance in astrocytes and leptomeningeal cells as a consequence of protozoan infection. Brain Research, 790(1-2), 304-314. https://doi.org/10.1016/S0006-8993(97)01523-0