Gap junction and tissue invasion: A comparison of tumorigenesis and pregnancy

E. Winterhager, B. Reuss, P. Hellmann, David C. Spray, R. Gruemmer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

1. Trophoblast invasion during embryo implantation in some aspects resembles tumour cell invasion but, unlike tumour cells, trophoblast cells are able to differentiate and establish a placenta, Because direct cell-cell communication is believed to be involved in growth control and differentiation, we have investigated connexin (ex) gene expression during trophoblast development. 2. Pre-implantation embryos expressed cx43 as well as cx31 proteins from the 8-cell stage onwards, Following implantation, compartmentalization of both connexins occurred: cx31 expression was restricted to the invasive trophoblast cell population, whereas the embryo proper was characterized by cx43. Trophoblast differentiation was indicated by induction of cx26 in the labyrinth and cx43 in the spongiotrophoblast accompanied by a disappearance of cx31. Comparison with trophoblast cell lines revealed that rat trophoblast HRP-1 cells express connexin43, while malignant choriocarcinoma cells express cx31. Treatment with retinoic acid led to a disappearance of cx31 in the choriocarcinoma. Both cell lines reduced their invasion properties after retinoic acid treatment, but growth retardation was only observed in the malignant trophoblast, 3. It seems that the cx31 channel is needed for trophoblast cell populations to maintain the highly proliferative properties but does not alter their invasion properties.

Original languageEnglish (US)
Pages (from-to)1058-1061
Number of pages4
JournalClinical and Experimental Pharmacology and Physiology
Volume23
Issue number12
StatePublished - 1996

Fingerprint

Gap Junctions
Trophoblasts
Carcinogenesis
Pregnancy
Connexin 43
Choriocarcinoma
Tretinoin
Trophoblastic Neoplasms
Cell Line
Connexins
Inner Ear
Growth
Cell Communication
Placenta
Population
Embryonic Structures
Gene Expression

Keywords

  • Connexin
  • Rat
  • Retinoic acid
  • Trophoblast

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Gap junction and tissue invasion : A comparison of tumorigenesis and pregnancy. / Winterhager, E.; Reuss, B.; Hellmann, P.; Spray, David C.; Gruemmer, R.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 23, No. 12, 1996, p. 1058-1061.

Research output: Contribution to journalArticle

Winterhager, E, Reuss, B, Hellmann, P, Spray, DC & Gruemmer, R 1996, 'Gap junction and tissue invasion: A comparison of tumorigenesis and pregnancy', Clinical and Experimental Pharmacology and Physiology, vol. 23, no. 12, pp. 1058-1061.
Winterhager, E. ; Reuss, B. ; Hellmann, P. ; Spray, David C. ; Gruemmer, R. / Gap junction and tissue invasion : A comparison of tumorigenesis and pregnancy. In: Clinical and Experimental Pharmacology and Physiology. 1996 ; Vol. 23, No. 12. pp. 1058-1061.
@article{35aa11fb695947bfb384ec171164b912,
title = "Gap junction and tissue invasion: A comparison of tumorigenesis and pregnancy",
abstract = "1. Trophoblast invasion during embryo implantation in some aspects resembles tumour cell invasion but, unlike tumour cells, trophoblast cells are able to differentiate and establish a placenta, Because direct cell-cell communication is believed to be involved in growth control and differentiation, we have investigated connexin (ex) gene expression during trophoblast development. 2. Pre-implantation embryos expressed cx43 as well as cx31 proteins from the 8-cell stage onwards, Following implantation, compartmentalization of both connexins occurred: cx31 expression was restricted to the invasive trophoblast cell population, whereas the embryo proper was characterized by cx43. Trophoblast differentiation was indicated by induction of cx26 in the labyrinth and cx43 in the spongiotrophoblast accompanied by a disappearance of cx31. Comparison with trophoblast cell lines revealed that rat trophoblast HRP-1 cells express connexin43, while malignant choriocarcinoma cells express cx31. Treatment with retinoic acid led to a disappearance of cx31 in the choriocarcinoma. Both cell lines reduced their invasion properties after retinoic acid treatment, but growth retardation was only observed in the malignant trophoblast, 3. It seems that the cx31 channel is needed for trophoblast cell populations to maintain the highly proliferative properties but does not alter their invasion properties.",
keywords = "Connexin, Rat, Retinoic acid, Trophoblast",
author = "E. Winterhager and B. Reuss and P. Hellmann and Spray, {David C.} and R. Gruemmer",
year = "1996",
language = "English (US)",
volume = "23",
pages = "1058--1061",
journal = "Clinical and Experimental Pharmacology and Physiology",
issn = "0305-1870",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Gap junction and tissue invasion

T2 - A comparison of tumorigenesis and pregnancy

AU - Winterhager, E.

AU - Reuss, B.

AU - Hellmann, P.

AU - Spray, David C.

AU - Gruemmer, R.

PY - 1996

Y1 - 1996

N2 - 1. Trophoblast invasion during embryo implantation in some aspects resembles tumour cell invasion but, unlike tumour cells, trophoblast cells are able to differentiate and establish a placenta, Because direct cell-cell communication is believed to be involved in growth control and differentiation, we have investigated connexin (ex) gene expression during trophoblast development. 2. Pre-implantation embryos expressed cx43 as well as cx31 proteins from the 8-cell stage onwards, Following implantation, compartmentalization of both connexins occurred: cx31 expression was restricted to the invasive trophoblast cell population, whereas the embryo proper was characterized by cx43. Trophoblast differentiation was indicated by induction of cx26 in the labyrinth and cx43 in the spongiotrophoblast accompanied by a disappearance of cx31. Comparison with trophoblast cell lines revealed that rat trophoblast HRP-1 cells express connexin43, while malignant choriocarcinoma cells express cx31. Treatment with retinoic acid led to a disappearance of cx31 in the choriocarcinoma. Both cell lines reduced their invasion properties after retinoic acid treatment, but growth retardation was only observed in the malignant trophoblast, 3. It seems that the cx31 channel is needed for trophoblast cell populations to maintain the highly proliferative properties but does not alter their invasion properties.

AB - 1. Trophoblast invasion during embryo implantation in some aspects resembles tumour cell invasion but, unlike tumour cells, trophoblast cells are able to differentiate and establish a placenta, Because direct cell-cell communication is believed to be involved in growth control and differentiation, we have investigated connexin (ex) gene expression during trophoblast development. 2. Pre-implantation embryos expressed cx43 as well as cx31 proteins from the 8-cell stage onwards, Following implantation, compartmentalization of both connexins occurred: cx31 expression was restricted to the invasive trophoblast cell population, whereas the embryo proper was characterized by cx43. Trophoblast differentiation was indicated by induction of cx26 in the labyrinth and cx43 in the spongiotrophoblast accompanied by a disappearance of cx31. Comparison with trophoblast cell lines revealed that rat trophoblast HRP-1 cells express connexin43, while malignant choriocarcinoma cells express cx31. Treatment with retinoic acid led to a disappearance of cx31 in the choriocarcinoma. Both cell lines reduced their invasion properties after retinoic acid treatment, but growth retardation was only observed in the malignant trophoblast, 3. It seems that the cx31 channel is needed for trophoblast cell populations to maintain the highly proliferative properties but does not alter their invasion properties.

KW - Connexin

KW - Rat

KW - Retinoic acid

KW - Trophoblast

UR - http://www.scopus.com/inward/record.url?scp=0030455143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030455143&partnerID=8YFLogxK

M3 - Article

C2 - 8977160

AN - SCOPUS:0030455143

VL - 23

SP - 1058

EP - 1061

JO - Clinical and Experimental Pharmacology and Physiology

JF - Clinical and Experimental Pharmacology and Physiology

SN - 0305-1870

IS - 12

ER -